XB-CLONE-4350121
Clone Name: IMAGE:7025773
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Gene:
ywhaq
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785726 [+]
taattaatccccccccccccctgtctgccggccgcgttgggtgaggagcgcaaggttacagctgtctgcgcatcagattcacctagaacgcctcttgttg
ttggttggcagacttcaacttttccctcctttccacctatctgatttcctccctcagcacagccatggacaggaacgcgcaaatccagaaggccaagcta
gcagagcaggctgagcgttacgatgacatggcggctagcatgaaagccgtaacagagtgcggcacagagctgtccagtgaagagagaaatctcctttctg
ttgcctacaagaatgtagtgggtgcaagaagatctgcctggcgtgtcatctccagcattgagcagaagtcagacagtgaagaaacaaagctgaagattgc
cagagagtacaaagagaaagtggagagtgagctacaaaatatttgcgaaactgttctgaacctgcttgacaagcacttaataagctcttcaactgcaaca
gaaagccaggtcttttaccttaaaatgaagggtgattactatcggtacctagcagaggtggcaactggagataaccgaactaaaacaatacaagattcac
aagctgcctatcaagaggcatttgacatcagcaaaaaggacatgcagccaacacatcccattcgcttgggtttagcctttgaatttctccgtgttttatt
atggaaatttctccaatagatccaagaaaaaggccctggtacacctcggcttaaaaaaacgcggttttgaatgaa
3' EST: CF785725 [+]tttttttttttttttgagtgtaatgacaatttattgatttactgaaggaggcaaatgttgtcaactgaagncagcaaacattggaaaaaatagtcaagtt
atccacaaacagtcaggagccgctgatggatgatgacgtttaacacttgccaactgcaaaactggttcaacaaaaaaaaaaaaaaaaaggcagcccaatt
tataataaagggtcctatccatctggtagtacaccagacgtctgacatttgtagttcttaaaattggaaggtaaccactggcaatttaccaccgtcattt
tgtgtttgttctcccccccccccccacagggcaaaatggtctttaaaagctacaagaaaataaaaaaaaatatatatgacagacatatttggattgaaca
gttaactacatttccctcacgctttattttaccatatatatataaaaaaggattttttctgaaaagcatagttccaattttttatttaaataccacacag
gctttcagtcctcaacataagtgttccaagttacttatgtttaattctcatttgtttttttgttgtttttaaacgaggttaaactgttaatacttctcta
cagcagtaaccacacaccagaaggatggacacattaaaagggagggggggaaaaaaaaaaataaaaaagggaatggaatttccccaaagctgcagtgtga
aaagactataaacttgatttccattacacatggaatgggccttcttttggctctaagaaaatccccgtggaagcgtaggaaatggaaacttgtaaaaagg
ggttccgggaaaaaagaaagtgcccagtttgctcacctttaatggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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