XB-CLONE-4350109
Clone Name: IMAGE:7025769
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Gene:
khdrbs1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785734 [+]
taattaatcccccccccccccggattttcttatcgacaccagcactgagcgtattgccgaccagtaacaccgaaccgagcaacatggagaccgagaccaa
atacttacccgaactgatggctgagaaggacagcctggacccatctttcacgcacgctatgagcctcctgggcaaggaaattgaaaggctgaagaaggga
ggtgatgccaagaaagatgaagaagatacttatctggacttattttcccataaaaacatgaagttaaaagagcgcattttgataccagtcaagctgtatc
ccaagtttaactttgttggaaagatccttggaccacagggaaacaccatcaaaaggttacaggaggagactggagcaaaaatctctgttcttggcaaggg
atcaatgagagataaggcaaaggaagaagagctgcggaaaggaggggaccccaaatacagccatctgaatatggaccttcatgtgttcatagaggtcttt
ggaccaccatgtgaatcttatacacgtatggcacatgcaatggaagaagtcaagaagttcttggttccgctgacacctgagtctttttcataccggacat
gatggatgatatctgtccggaacagtttatgggagctatcttatcttaatggagccccccccagaccaaagccgaggtgggaacaggaagtggaaccatt
ccaggggcagaagggggtcccacaacctggcgggagcttccttttttcctaaaaggcaggggctggggccctctttcgcccctcctttgtgttccaaaaa
agggtgggccccccgggat
3' EST: CF785733 [+]tttttttttttttttcttgtccataaggtttaataacataaagtggattttaagtacaaagtcaaaagtttttgttttaaacagcatacaatattacagc
tacaattttaactactcaaagtaatgggattttgaaatgcagcttagttcaggacaaagaacctaaagctatagttacttttgtttatttaaagaaactt
gttttgaagtcactttgaaaaactaccctttaatgcagctaaattttacatgtaaagcattttgtattttttaaagtttttgtttctttaacaacgcaaa
gtaaaacgatctaaatattaaaaaaaagtctgcctatttcagagttgctccatttttttttttaaaattgtcctttcaaaggaatttgggaaaagggagc
tatatgagggaggtcacagagcacagagaatttacttgtgtattgtgggttaaggaacaggaagtcccgaacacaagacatcggtaaacatggtttggat
gaaaagatgttcaaaagcgcccataaggatgttctctgtatgcaccgcccttcaaaggacgtgaaaagggaaccttaagtgaaagtcgggatccattcca
attatcttgtccataactctcatacgaattcggaccctctccatgaccataatcctaaatattccgtctctccttgggctttgaactgtaaaaatccctc
aaaaaccttcaaagcttggcctctgcataaggaatccttcaaaaccctatttcaccaaaaggttttccgggggccggaaggtgggaaaaaaaaattcctt
a
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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