XB-CLONE-4350099
Clone Name: IMAGE:7025765
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Gene:
ca2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785740
[+]
taattaatcccccccccccgagagctcttgggcacccagaaagggacacttgtcacaagcaggcagcgagctgacagcacacaactactccacacagaat
ggcacacgcttggggatatggtcctgacaatggaccagcaacttggcaccatgccttccctattgcccatggagatcatcagtcccctattaacattgta
tcagccgaagctaagtatgaccaccaccttaagcccataagcatcaagtacgacccgtccacggccaaagtcatcctgaacaatggacacgccttcaatg
tggagttcgacgactcagaagacaaatcagtgcttagtgggggagcagtgagtcacccctaccgccttaagcagttccacttccactggggatcttgtga
cggtcatggctcggagcatacagtcgatggagtcaaatatgaagcagagcttcacttggttcactggaatacaaaatatgccagcatggcagaagccgta
aaacactgtgatggacttgctgtggtcggggtctttttaaaggtaggagaagcacatccaggcttgcagaaagtacttgatgcactaaagcttataccaa
acaggataacgaggcaccctttactgattacgatccttctgtctttacttccaattcactggattttctggaattataaaaggatcttttgactactccc
ccccattgctcccaagtgtgtttctgttggcatgtccctgaggggaaacacattagcttttgaagcaaatcaagcaagctggtcaacaggcttgcgttag
cccctttttcttttcaaag
3' EST: CF785739
[+]tttttttttttttttgcaagtataactttttattaaggtatccaaaatacacatatatttatatatatttatatatatatttattgtaatatataattca
tctgctagttgaagttaagacaggtgcaaaataagcaccattattaagacatttaatataagctatataaatgtaaaatctgtatacctctcttaaaagc
gatgtctgaataatccaaactgataaacaatttgaaaatcagttcacattttttaatacatatcagtgtatgtacttaaattcaagtttctttattaagc
agagaagcctacaacaattctaggagctctagggaggaattcacggatgtcaaacttataatttgttatattttaccattaaaatattgcctcttttttc
aaaattgtaaaattgcctgtttaaaaagcaaaaatatccacgttcaaaaagttgcaaatgatgtttgttagttaaccccaaaccttacataacccaatta
aaaaaagtccccctgtggccccacacccttaattttccattttgcccaatttttttgggggggtttttttcccggggtttttaatttggcttaaataaaa
atttgggtaaaaaatttaagggtttttttttttttccccttaaaaaaaaaaaaaaatttttttttttttgaaaggaaaacaatcccccggggggggtttt
tttggggggaaaaaatatttttcccccctctttttcccccccaaaaaacctttggggttaaaaaaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: