XB-CLONE-4350096
Clone Name: IMAGE:7025764
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Gene:
capns2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785741
[+]
taattaatcccccccccccccgagagaagctgtgcgaacgtcccaacaccaaaagttttaggttcatacaaccatgttccttataaacaacttcatggga
ggtggcggaagaggtggtggaggttttggtggatttggaggtggatctggtcttataggaggaattttaaattccctcagtgaagctgctgcaaactaca
atccacagcctccgcctccacggtctcatccctctgttattcaatcaaatgaaagtgaagagaccagacagtttcgacgtctgtttagtcaactagcagg
agatgacatggaagtcagtgccacagaactgatgggaatccttaacaaagttatagcaaaacatcaggatctgaagacagatggatttagtgctgattca
tgtcgcagtatggtggctgtaatggatagtgacagcacagggaaacttgggtttgaagaattcaaatacctatgggacaacataaaaaaatggcagggtg
tatataaaagatatgacactgatcgttctggcactattggaagaaatgagctccctggagcatttacagcagcaggctttcagttaaatggccagcttta
tgatatgattatccgtcgttattctgatgagaaaggagacatggattttgataactatatctgttgcttagtgcgacttgatgccatgttccgtgctttt
aaaactctggataaaagatggagatggccagattaaagttacaattcaggagtggctacaacttactatgtattcatgagaagtcacttgtgtggtcttg
gacctgtgggagccctaaagttactn
3' EST: CF785742
[+]tttttttttttttttgtttacagcttttaattgcatccatttttcataacagagtttgagaaaggttcaccttaaaacaaattttccataaagaatgtaa
caaatttgatttatttattgatagaactggtgccattatctagatgtatgcatacagaatataaaaatctttcatacatacttttgcatacacatgcaca
cacaagctgttacgtgttgcctctggtgctcaaatttaaaattctgtaatatagtagaagtctctttccgttacaatatataggataggtatatattttt
cacttataaaacaagccttgtatacagcacacagttatttagtacagttggggaaaaaagtaactttagggctccacaagttcaagaccacacaagtgac
ttctcatgaatacatagtaagttgtagccactcctgaattgtaactttaatctggccatctccatctttatccagagttttaaaagcacggaacatggca
tcaagtcgcactaagcaacagatatagttatcaaaatccatgtctcctttctcatcagaataacgacggataatcatatcataaagctggccatttaact
gaaagcctgctgctgtaaatgctccagggagctcatttcttccaatagtgccagaacgatcagtgtcatatcttttatatacaccctgccatttttttta
tgttgtcccataagtatttgaattcttccaacccaagtttcccctgtgctgtcactaacccttacagccaccatactggcgaca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: