XB-CLONE-4350093
Clone Name: IMAGE:7025763
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Gene:
dnajc15
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077694 [+]
CCGGGCTATCTTCCTCCTACTGTGCTTCTGGCAGAGACTCCACCGTAAGTCTTACTGTCATGGCGACGTCCAGCACCGGCATGGGGACATCTGAAGCGCT
GCGCTATTCTGAGTATATGCCAGGTCCGAATGCGGGCTCATCCACTGATATTGATCGACGACTGGGTGGAACATTAATAGCGGTGGGACTTGGTGTTGCA
GCTGCGGCTTTTGCAGGTCGCTTTGCATTTCAGCTGTGGAAACCTTTGGGTCAAGTTATAGCAGAAAGCGCTAAAAAAATCCCAACCCCAAGCCTTTCTT
ATTATTACAAAGGAGGATTTGAACAGAAAATGAATAGGCGGGAAGCTAGCCTTATTCTAGGTGTCAGTCCGTCTGCTAGCAAATCCAAAATTCGCGCAGC
TCATCGAAAAATCATGATTTTAAATCATCCAGATAAAGGTGGATCTCCTTATATGGCAATGAAGATCAATGAAGCAAAGGACTTACTGGAATCCACTACC
AAACCTTGATAGAGATAAATTATAACTTGCATTAGGCCTGTTACTAATTGATGTGTTTCAGTGTAAAGTATTTATGCTGGTTATTTGCACTTCCAGACTT
CTTTTTATTCCAAGTTCCAACTATACCTGTATTTATTTTGAGGAAGTTTCAGGGACAAGCCATTTAGTTTCAGGGTCCCAAGAACTGGATGCTATTGGTG
ACGCTTTTGTCAGATACACAACTGTCCTCTAGAAACCTCTAAAATATGGCATTGCCATTTCTTATAGTATCCTACTGTAAGCAGTTCTTCATATGAGATT
GGTTTGCTTCTTTATCTTTAATAATGACAGTACATACCAGTTGATGTTAATATAAGTATCATTATGATGATAAACAGTTTTGGGTTTTTTCATGTTCAAA
ATATTCAAAACCTTTTTTTATACAGAATCCCATACTTTCCATAGATTATTTTGAATTAGCAGTGTTTTTATGCACTGCAACATGGAACAATATTGAATTC
AGGATTTATGCAGATGTGTTCCAGCACTTTGCATCTGTGCCAAGGCCAAATCCTTTCTATGTAAGCTGGAAAGAATGTACAAAACACAGTTCCAGAGCTG
TAGATACTCTTGGGTGACAATGCTAGCAACTACAATAAATGCAATTAGGAGGCTTCAATCTTCTCTATAATTTGGACCTCTGCTGTTTCTGCACCAGCTC
CCATACAATTTGAAACATTAAATAAGTAATAAAAAAAAAAAAAAAA
5' EST: CF785744 [+]taattaatcccccccccccccccgggctatcttcctcctactgtgcttctggcagagactccaccgtaagtcttactgtcatggcgacgtccagcaccgg
catggggacatctgaagcgctgcgctattctgagtatatgccaggtccgaatgcgggctcatccactgatattgatcgacgactgggtggaacattaata
gcggtgggacttggtgttgcagctgcggcttttgcaggtcgctttgcatttcagctgtggaaacctttgggtcaagttatagcagaaagcgctaaaaaaa
tcccaaccccaagcctttcttattattacaaaggaggatttgaacagaaaatgaataggcgggaagctagccttattctaggtgtcagtccgtctgctag
caaatccaaaattcgcgcagctcatcgaaaaatcatgattttaaatcatccagataaaggtggatctccttatatggcaatgaagatcaatgaagcaaag
gacttactggaatccactaccaaaccttgatagagataaattataacttgcattaggcctgttactaattgatgtgtttcagtgtaaagtatttatgctg
gttatttgcacttccagacttcttttttttccaagttccaactatacctgtatttatttttgaagaaatgtttcagggaccaagccatttagttttccgg
gtcccaagaaactggaaggcttattgggtggacgccttttttgtcggaaaacaccaaccgtgtcctccctaaaaaaaccctcctaaaaaaatggggc
3' EST: CF785743 [+]tttttttttttttttattacttatttaatgtttcaaattgtatgggagctggtgcagaaacagcagaggtccaaattatagagaagattgaagcctccta
attgcatttattgtagttgctagcattgtcacccaagagtatctacagctctggaactgtgttttgtacattctttccagcttacatagaaaggatttgg
ccttggcacagatgcaaagtgctggaacacatctgcataaatcctgaattcaatattgttccatgttgcagtgcataaaaacactgctaattcaaaataa
tctatggaaagtatgggattctgtataaaaaaaggttttgaatattttgaacatgaaaaaacccaaaactgtttatcatcataatgatacttatattaac
atcaactggtatgtactgtcattattaaagataaagaagcaaaccaatctcatatgaagaactgcttacagtaggatactataagaaatggcaatgccat
attttagaggtttctagaggacagttgtgtatctgacaaaagcgtcaccaatagcatccagttcttgggaccctgaaactaaatggcttgtccctgaaac
ttcctcaaaataaatacaggtatagttggaacttgggaataaaaaaaagtctggaagtgcaaataaaccagcataaatactttacactgaaacccatcaa
ttagtaacagggcctaatgccaattaaaatttatctcctatcaagggttgggtaatggaattcccgtaaatcccttggccttcattgaccttcctg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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