XB-CLONE-4350087
Clone Name: IMAGE:7025761
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Gene:
ppp5c
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080162 [+]
GACAGTAACCGGCAGCGGGAACATGGCAGAGGCAGAGCGAGCTGAGGAGGCCGGAGGAGAACCAACAATTAGCAGGGAGAAGACTGCTGAAGAGCTAAAG
GAACAAGCGAATGAATACTTCAGGGTAAAAGATTATGACCGTGCTGTGCAGTATTACACCCAAGCCATTGGTCTTAGTCCAGACACTGCTATTTACTATG
GGAATCGCAGTCTGGCATATCTCCGAACTGAATGTTATGGCTATGCCCTTGCGGATGCTTCCCGTGCCATCCAGCTGGATGCCAAATACATCAAAGGTTA
CTACAGAAGGGCAGCCAGTAATATGGCCCTGGGGAAGCTGAAAGCTGCTCTAAAGGACTATGAGACGGTAGTTAAAGTTCGCCCTCATGACAAAGATGCC
CAAATGAAGTTTCAAGAGTGCAGTAAGCTGGTCCGGCAGAAAGCTTTTGAAAGAGCTATCGCTTGTGAACAGCACAACCGTTCAGTGGTGGACTCACTTG
ATATTGAAGGAATGACTATAGAAGATGAGTATACAGGTCCACAGTTAAAGGATGGGAATGTCACCATAGATTTTATGTTGGAGTTGATGCAGTTTTATAA
AGATCAGAAAAAACTTCATCGGAAATGTGTTTATCAGATGTTGGTACAAGTGAAAGATATTTTGTCTAAGCTTCCCAGTCTGGTAGAGATCTCTCTGGAA
AAGTCTCAGCAGGTAACAGTGTGTGGTGACACACATGGTCAGTTTTACGACTTAATGAACATTTTTCATCTAAACGGTCTCCCGTCTGAGAATAATCCAT
ATATATTTAATGGTGACTTTGTTGATCGTGGTTCATTCTCAGTGGAAGTCATAGTGACCCTCTTTGGGTTCAAATTGCTTTATCCAGCCCAATTTCATCT
GCTTAGAGGTAACCATGAAACAGACACTATGAATCAGATGTATGGATTTGAAGGGGAAGTAAAGGCTAAATACTCAGCTCAAATGTTCCAGCTATTTAGT
GAAGTTTTTCAGTGGCTGCCGTTGGCAATGTGTGTAAACCAACGAGTCCTGATTATGCATGGAGGTCTCTTCTCAGAGGATGGTGTAACTTTGGATCAGA
TTCGTAGCATTGAAAGAAATAGACAGCCACCAGACTCAGGCCCAATGTGTGACTTGTTATGGTCGGATCCACAGCCTCAGGATGGACGCTCTTCTAGTAA
ACGTGGTGTCAGCTGTCAGTTTGGTCCTGATGTCACACGACGGTTTTTGGAAGAAAATGGACTAGATTACATTATCCGCAGCCATGAAGTGAAGCCAGAA
GGATATGAAGTGTCTCACAATGGTTTATGTGTTACAGTATTCTCTGCACCTAACTACTGTGATCAGATGGGTAATAAAGGAGCATACATACATCTCAATG
GATCAGATTTGAAACCTAAGTTTCATCAGTTTGAGGCAGTGCCACACCCGAATGTAAAACCAATGATGTATGCAAATTCTCTGCTTCAGATGGGAATGAT
GTAAGAAGAAGGTGTCTGCTGCTACCACACTTACTGTTGCCCATCTGATGAGGTAGTAAAGGGGTCCCACTGTATTTGCCCCTGCTTTCCTCCGGAGTCA
GACAAGCAACAAATAAAGGAAAATAATATTTTTAATACTGTAAAAGAGACATGAGAGGGGGAATAGGAACATCTGCGCTGTATGTTACCTGGTTGTGAGT
GAACCTTGCTGTGTGTTGCTGAGTGAGTGTGAATATAACAGTGTAAGTGTGGGGGGGTTTATCAGCTAGAAACGCACAATAACCACACCGCCCCCCATTC
CTATATGCCTTGTGCGTACACTCTCCCTCCTGTATAAGTCCAATGTAAACATGGCCCCTTTGCTCTGTCTGTAATTAATATGCTTCTAATAAAGATTCTG
ATAACACGAAAAAAAAAAAAAAAA
5' EST: CF785748 [+]taattaatcccccccccccccgacagtaaccggcagcgggaacatggcagaggcagagcgagctgaggaggccggaggagaaccaacaattagcagggag
aagactgctgaagagctaaaggaacaagcgaatgaatacttcagggtaaaagattatgaccgtgctgtgcagtattacacccaagccattggtcttagtc
cagacactgctatttactatgggaatcgcagtctggcatatctccgaactgaatgttatggctatgcccttgcggatgcttcccgtgccatccagctgga
tgccaaatacatcaaaggttactacagaagggcagccagtaatatggccctggggaagctgaaagctgctctaaaggactatgagaaaggtagttaaagt
tcggccctcatgacaaaagatgccccaaaatgaagtttttccaagagttgtcaaataaagcctgggtcccgggcaaggaaaagctttttttgaaaaagaa
gctcaatcggacctgtggtgaaaaccagcccaccaaagcccggtttacaagaagggattggaggacgttcccacgtcttggacctagttttggaaaacga
ggaatatttggcaactcttattaatatagataaggacatatggttatgcatcaagttaaccctgagggggggtttactccaatcaccacgggtctttttt
cgatgcaaacgggggggtaaatttttggggggggttgagaaacagatggggggtgcctacctagccccccgtctagtggtaagagagaatatatgttttt
ctattgaccggggagttttatagggggggaaagagttgttttgcagaaaacttgctgcccccgggggctgttctgtaattgcacactaaaagaaaaggga
tatttttccacaagatattgctaatttcgctcaaacccgtttgtttcacgtccattgtcatggtaacaanaaaatggggggtgtgttttttcactcgttt
cccccaccaaaaatgtgtttttcggaggggtctatcgcacaagattgcggcgagaaaaaggagactcactttatattagggggccgccaaagaggatcct
agtgttcccgccaaagactcattgtgaggactaggaagcaaattatgtcagcgtctctgagggaaaaaaaaaatcgcctggcagttgaaatgggtgtgca
ataacaataagtgagtagtgccaaaaccctcacacatg
3' EST: CF785747 [+]tttttttttttttttcgtgttatcagaatctttattagaagcatattaattacagacagagcaaaggggccatgtttacattggacttatacaggaggga
gagtgtacgcacaaggcatataggaatggggggcggtgtggttattgtgcgtttctagctgataaacccccccacacttacactgttatattcacactca
ctcagcaacacacagcaaggttcactcacaaccaggtaacatacagcgcagatgttcctattccccctctcatgtctcttttacagtattaaaaatatta
ttttcctttatttgttgcttgtctgactccggaggaaagcaggggcaaatacagtgggacccctttactacctcatcagatgggcaacagtaagtgtggt
agcagcagacaccttcttcttacatcattcccatctgaagcagagaatttgcatacatcattggttttacattcgggtgtggcactgcctcaaactgatg
aaacttaggtttcaaatctgatccattgagatgtatgtatgctcctttattacccatctgatcacagtagttaggtgcagagaatactgtaacacataaa
ccattgtgagacacttcatatccttctggcttcacttcatggctgcggataatgtaatctagtccatttttcttccaaaaaccgtcgttgtgacattcag
accaaactgaaagctgaacccaccgtttactaaaaagagcggtccatcctgaaggctgtgggatccaaaccataacaggttccactt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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