XB-CLONE-4350075
Clone Name: IMAGE:7025757
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Gene:
myl6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785756
[+]
taattaatcccccccccccccgacattccggaagggagaagacgcgtcaagatgtgcgactactccgacgaccagattgccgactacaaggagtccttcc
aactttttgaccgtgtcggcgatggcaaaatcctcttcggccagtgtggggacgtcatgcgggcactggggcagaaccccactaatgctgaggtgatgaa
ggttttgggaaacccaaagcctgaagacatgaacatcaagacacttgacttcgagcaattcctccccatgatgcaaacagttgccaagaacagggatgta
cctggattggaggacatcatagaagggctaagagtcttcgacaaggagggaaatggcacagttatgggctccgagttgcgtcatgtcctggtttccctcg
gagagaagatgacagatgatgaagtagagacccttttaagtaatcatgaggatgctaatggttgcattaattatgaagaacttatccgggcgatattgaa
cggctgaagcttctcctttagtttacctctgcccagtggggatgaagtgtgggggggggaagttagttagcaatttattcaagcccttcactagagagga
agacaggctatatagtaagtgccttttctgcttcctccttatcccatcagtcacccccttcttctcgcaagtggcaaaattcgtattcattttttctgta
acatcacaagggaggcccacagctacagctttaatgaacatcggtagaaattgtgggcaaaataattttttcatatcaacccccccccccccccaggctt
tccttt
3' EST: CF785755
[+]tttttttttttttttggaaaaagtcactcagtttatttgatgttggataggggaaaaaatagaaatttgaaataatttatctttctctgcacactacagc
attaaggaagcatgggggggggggggtgatatgaaaaattattttgccacaatttctaccgatgttcatttaagctgtagctgtgggcctcccttgtgat
gttacagaaaaaatgaatacgaattttgccacttgcgagaagaagggggtgactgatgggataaggaggaagcagaaaaggcacttactatatagcctgt
cttcctctctagtgaagggcttgaataaattgctaactaacttcccccccacacttcatccccactgggcagaggtaaactaaaggagaagcttcagccg
ttcaatatcgcccggataagttcttcataattaatgcaaccattagcatcctcatgattacttaaaagggtctctacttcatcatctgtcatcttctctc
cgagggaaaccaggacatgacgcaactcggagcccataactgtgccatttccctccttgtccaagactcttagccctttctatgatgtcctcccaatcca
ngtacatcccctgttctttggcactgtttgcatcctgggggaagaaattgctccaagttcaagtggccttgaaggttcagggtcttcaaggcttttgggg
tttcccaaaacctttcatcacctcagaatttaatggggggttcttgcccccatgggcccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: