XB-CLONE-4350072
Clone Name: IMAGE:7025756
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Gene:
darmin
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785757 [+]
acaaggctcaaggagagatctgcactctggtggctttggagggacagtacatgaagctatgagtgatttgatatatttgctgaacacgctggcagatgga
aagggtcgcatccttgtcccagggatttatgaggctgtggcacctgtgagtgagaatgaaacagatttgtacaaaaatcttgaattcagtcaacaggaaa
tgcaagctgacacaggggtcacgcaattccttcatgatacaaaagaagatttacttatgcatagatggcgctatccttcactaacaattcatgggattga
gggggctttctgtggaacaggaactaaaactgtaatccctgcaaaagtaattggaaagttctctatgcgtcaagttcctaacatggatccatctgttgta
gaaaaacaggttactgattacttggaggccaaattttctgaaaggaaaagccctaataaaataaaggtaaaaatggtaattggagcaaaaccttggttgg
cagacatgaatgagccacaatatctggctgctagaagagcagtaaaaagagtgttcaacctggaagcagacatgatccgagctggtggaactattcccat
tgctaaaactttggaagatgtacttggaaagagtgtaatgctactaggaattggtggaccagatgatgctcctcatggtcaaaatgagaaaataagcaag
tacaattacattggaagtaccaaactgtatgctttcttaccttcaa
3' EST: CF785758 [+]tttttttttttttttggtgttacatatttaatttaaaaatatctatacagagaaagactcatcggttgctgattactgttagacacagttcagatggctg
ttgaagaaagttcttgaaggtaagaagcatacagtttggtaccttcaatgtaattgtacttgcttattttctcattttgaccatgaggagcatcatctgg
tccaccaattcctagtagcattacactctttccaagtacatcttccaaagttttagcaatgggaatagttccaccagctcggatcatgtctgcttccagg
ttgaacactctttttactgctcttctagcagccagatattgtggctcattcatgtctgccaaccaaggttttgctccaattaccatttttacctttattt
tattagggcttttcctttcagaaaatttggcctccaagtaatcagtaacctgtttttctacaacagatggatccatgttaggaacttgacgcatagagaa
ctttccaattacttttgcagggattacagttttagttcctgttccacagaaagccccctcaatcccatgaattgttagtgaaggatagcgccatctatgc
ataagtaaatcttcttttgtatcatgaaggaattgcgtgacccctgtgtcagcttgcatttcctgttgactgaattcaagatttttgtacaaatctgttt
cattctcactcacaggtgccacagcctcataaatccctgggacaaggatgcgaccctttccatctgccagcgtgttcagcaaatatatcaaatcactcat
agcttcatgtactgtccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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