XB-CLONE-4350067
Clone Name: IMAGE:7025754
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Gene:
slc3a2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081372
[+]
GGTCACACAAAGCCCTTACTGACAGCTGCCTGGAGCGTGAGCACAAGACACCAGGACACCGACATGACTCAAGATACCGCCCTAGACATGAAAGACGTTG
AACTGAATGAATTGGATCAAGAGAAGGTCCCAATGGCAGGTGGGGCTGGGGACAGCCCAACTGGAGGGGGAGAGAAGAATGGCGTTGTTAAGGTGAAGCT
TGAAGATGATGATGCGATTGGAACCAAGAGTCAGAAATTTACAGGGTTGTCTAAAGAAGAATTACTGCGAGTAGCTGGTACCCCGGCATGGGTGAGAGTC
CGATGGGCATTGCTGATCCTGTTTTGGTTAGGCTGGGCTGGCATGCTAGCAGGAGCTGTGGTCATCATTGTACAAGCTCCTCGCTGCCAGCCTTTGCCAG
CAATGGAATGGTGGAACAAGGGACCCCTCTACCAAGTTGGAGATCCAGCAACCTTTCAAGAAGATGGGGCAGGAAATATTCAGAGTATAGAGAAGCGCCT
TGAATCACTTACCAGTCTGAAAATAAAGGGTTTGATTATTGGTCCAATACATGCTACAAAGAAAGATGAAATTGACAAAACCATACTCACTGATATTGAC
CCTAATTATGGCACACAGGAGCAATTCACCAGTCTGCTGGAAGCTGCGCGCAAAAAGAGTATCCAGATCATCTTGGACCTCACTCCTAATTACCGCAGTG
AAAAAAGCTGGTTTGAAAAGACTGACATTAACTTTGAGGATAACGTAAAGGAAGCAATTAATACTTGGCTGGAACGCGGTGTCGGAGGTATATACTTTGG
AGACAGTGAGAATTTGCCCAATACAAGCAACTTTATATATGAATGGGGGAACATGACTGCCAATTTCAGCAAAGAAGGAAAACCAAGAGTCCTGCTGTTA
TCCTCAAATAGTACTCAAAACCAGCTTGCTGGCAACTTCAACGAGACTGATGGCATACTCTTCTATCGCTTCCTGGATGCTGAGAACAAGAAAAGCTTTA
GATCTTTGGGTGGGGACATCAAGCAGTATGTGGAAGAAACTGGCATCCTGGGAAACAGTTAGATGATTGGAGCACCACAAATGGGCCATATGGCTTCTTT
GGTCAACGAAAAGCTATTTCGTGTGTACCAGCTGCTCCTCTTCACACTGCCAGGCACACCAATCTCATTGTACGGGGATGAAATTGGACTTAAAGACCTT
CCTAGCAAGCCTGCTCAGTCTTCAAGACCTAACATGCAGTGGGATGAAGTTTCAGTGGCTGCTAATTCCCCACAAATCTTATCCGATGTAAATGCAAATG
TCACATTTAAGGCACAGGATACTGACAAGGGGTCGTTCCTAAACATATACAGGAAATTGAGTGACCTGCGTGGGAAAGAACGATCCCTCCTGCATGGGGA
ATTTGTATTACTGTATAACAGTGACAAGGCCATAGCTTTCCTAAGGAGCTGGGACCAGAATGAGCGATATGTGACAGCCTTGAACTTTAACTATGAGGGA
GAGGTAGAACTCTCCTTGAAAAAGGACAGAGGCGAAGAGCTGCCAGAACACGGCACAGTAGTGTTGAGTTCCAGCTCCCAACGAAAAGAAGGGGAAAGTG
TTTCCCTAAAAAGCTTGCAGTTAGGAGCTGGAGAGGCTTTACTCCTTAAATACCCTTATAGTGGATAACCCCCTACATCCCTGTGAGGGGCAAACACTTA
CACATTCCACTTGCTGACCCTTCTTTCCAGTGCCAAGAAGCACATGTTGTTAACCTTCCCTTTAAACCTTTTAACACATCCCTACCCTTTGTGGCAGTGG
CATATACTGTAACCTGCCATTTATATTTGCCAAGCGGTCATATTTTTCATGTTCAGAGGATAAAAACTTAAAAACAGTTTCTGAAACGCGTTAAGAGCAA
TAAGTGGAGTGTTGTTTTCTGCTTAGTTTTGTCATACACGTGCTCTGAGGTGTGTTTTTATGTTGTCATTTCATGTTTTAAAGGCTGTGGCACTTTTAGC
CCTCTGAGCATGGAGAGATGGGCCGCAGCCAGGGGAATATGAATGTCTGTGGATCTTGTGTATTTGGGGAGGGGAAAGTTTGGTTACAGTTGTGCAACAT
GGTGCTGGTCAGCAAAAAATTCCTACTTCAATAAAGTTAGTGTTTGTTTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF785760
[+]taattaatcccccccccccccggtcacacaaagcccttactgacagctgcctggagcgtgagcacaagacaccaggacaccgacatgactcaagataccg
ccctagacatgaaagacgttgaactgaatgaattggatcaagagaaggtcccaatggcaggtggggctggggacagcccaactggagggggagagaagaa
tggcgttgttaaggtgaagcttgaagatgatgatgcgattggaaccaagagtcagaaatttacagggttgtctaaagaagaattactgcgagtagctggt
accccggcatgggtgagagtccgatgggcattgctgatcctgttttggttaggctgggctggcatgctagcaggagctgtggtcatcattgtacaagctc
ctcgctgccagcctttgccagcaatggaatggtggaacaagggacccctctaccaagttggagatccagcaacctttcaagaagatggggcaggaaatat
tcagagtatagagaagcgccttgaatcacttaccagtctgaaaataaagggtttgattattggtccaatacatgctacaaagaaagatgaaattgacaaa
accatactcactgatattgaccctaattatggcacacaggagcaattcaccagtctgctggaagctgcgcgcaaaaagagtatccagatcatctttggac
ctcactcctaattaccgcagtgaaaaaagctggtttgaaaaaactgacataaacttttgagataacgtaaaggaaagcaa
3' EST: CF785761
[+]ttttttttttttttttttttttttttttttatgaaacaaacactaactttattgaagtaggaattttttgctgaccagcaccatgttgcacaactgtaac
caaactttcccctccccaaatacacaagatccacagacattcatattcccctggctgcggcccatctctccatgctcagagggctaaaagtgccacagcc
tttaaaacatgaaatgacaacataaaaacacacctcagagcacgtgtatgacaaaactaagcagaaaacaacactccacttattgctcttaacgcgtttc
aaaaactgtttttaagtttttatcctctgaacatgaaaaatatgaccgcttggcaaatataaatggcaggttacagtatatgccactgccacaaagggta
gggatgtgttaaaaggtttaaagggaaggttaacaacatgtgcttcttggcactggaaagaagggtcagcaagtggaatgtgtaagtgtttgcccctcac
agggatgtagggggttatccactataagggtatttaaggagtaaagcctctccagctcctaactgcaagctttttagggaaacactttccccttcttttc
gttgggagctggaactcaacactactgtgccgtgttctggcagctcttcgcctctgttcttttttcaaggagagttctacctctccctcataattaaagt
tcaaggctggtcacataatcgcttcattctgggtcccaagctcctttaggaaaaccaatgggccttgtgtcactgggttataacagataaataacaaatt
tccccccatgccaaggaagggaaatccgtttctttttccct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: