XB-CLONE-4350057
Clone Name: IMAGE:7025750
|
Gene:
vps29
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC077001
[+]
CCAATCAGTGACTGTTCCTCTTCTGTCTGAGCAGAAACGGAGTGCAGGATGTTGGTTTTAGTATTAGGAGACCTGCACATCCCTCACCGTTGCAATAGCC
TGCCTGCAAAGTTCAAAAAACTGCTTGTACCTGGGAAGATTCAGCACATTCTGTGCACGGGAAACTTATGCACCAAGGAAAGTTTTGACTACCTGAAGAC
TCTGGCTGGGGACGTTCACATTGTCAGAGGAGACTTTGACGAGAACTTAAATTACCCAGAACAAAAGGTTGTGACTGTTGGACAGTTTAAAATCGGTTTA
ATTCATGGCCACCAAGTAATACCATGGGGAGATATGGCTAGTCTGGCCTTGTTACAGAGACAGTTGGATGTTGATATCCTGATTTCTGGACATACACAAA
AATTTGAAGCATTTGAACATGAAAACAAATTTTACATCAACCCAGGATCTGCTACCGGAGCTTACAATGCATTAGAAAACAATATTATTCCTTCCTTTGT
ACTGATGGACATCCAGGCCTCCACTGTTGTCACCTATGTGTACCAGTTAATTGGAGATGATGTGAAAGTAGAAAGAATTGAGTATAAAAAATCATAAAAT
AGAACTGCCAACTGTTTCATGGCCTTAATTTTTTCATTCCATGAATTTAGCTAATGAGATGGTCATGACAAACCCTGCAAAAGCTATAGGCAGATAATTA
GCTATGTACTAATATTTGCATTATAACTTCCTCTGAATTTGCTGGAATAGGAATCTGTACATCAGATAATTTGTTCTTTTAAATGTGTTGCAAATCGTGT
GCCATGTTTATTTACTATGTGAAAAAAAAAAAAAAAA
5' EST: CF785766
[+]taattaatcccccccccccccccaatcagtgactgttcctcttctgtctgagcagaaacggagtgcaggatgttggttttagtattaggagacctgcaca
tccctcaccgttgcaatagcctgcctgcaaagttcaaaaaactgcttgtacctgggaagattcagcacattctgtgcacgggaaacttatgcaccaagga
aagttttgactacctgaagactctggctggggacgttcacattgtcagaggagactttgacgagaacttaaattacccagaacaaaaggttgtgactgtt
ggacagtttaaaatcggtttaattcatggccaccaagtaataccatggggagatatggctagtctggccttgttacagagacagttggatgttgatatcc
tgatttctggacatacacaaaaatttgaagcatttgaacatgaaaacaaattttacatcaacccaggatctgctaccggagcttacaatgcattagaaaa
caatattattccttcctttgtactgatggacatccaggcctccactgttgtcacctatgtgtaccagttaattggagatgatgtgaaagtagaaagaatt
gagtattaaaaatcataaaatagaactgccaactgtttcatggccttaaattttttcattccatgaatttagctaatgagatggtcatgacaaaccctgc
aaaagctatagggcaaaaattagctatgtactaatatttgcctttaaacttcccccgaatttgctggaaataggaatctg
3' EST: CF785767
[+]tttttttttttttttcacatagtaaataaacatggcacacgatttgcaacacatttaaaagaacaaattatctgatgtacagattcctattccagcaaat
tcagaggaagttataatgcaaatattagtacatagctaattatctgcctatagcttttgcagggtttgtcatgaccatctcattagctaaattcatggaa
tgaaaaaattaaggccatgaaacagttggcagttctattttatgattttttatactcaattctttctactttcacatcatctcccatttaactggtacac
ataggtgacaacagtgggaggccctgaatgtccattcattacaaaaggaaggaataaatattggttttctaaaggcatttggaaacctccgggaaaccaa
attcctggggttgaaggtaaaaattttgtttttcaggggtccaaaaggcttcacaaattttttggggggggggcccccgaaaatccccggggatttccaa
ccctccccaaaggtggtcccttgttaaaaaagggggcgccaaaaaaaacccccataatatcccccccccaggggggagaattatctggggggggggcccc
cgaaaatattaaacccccctttttttttaaaaagtggggccccaaaaaagtcgtccccaaaccctcttttttttttttttgggggggaagaaaaaataaa
aaaaatttcctccccgcaaaaaaaaaaatctccctcctccttttgtaaaaaaatggggttgggaagagacctccccccccccacccaaaaaaaaagaatc
ttttcgggggggggggagaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: