XB-CLONE-4350050
Clone Name: IMAGE:7025746
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785770
[+]
taattaatccccccccccccccatgtctttctgaaatgtgaattataattacctaggttaaatgatattcctttctgtgtatttgggatttgatttatta
ctaagcagcttatgatttattgaggagttatttgtaaccctgaagttcatttaccaacactgggcaaatttgcacctgggtaaaaactcacagcaaccaa
ttagtgattagcattgttcaaccagatacaggtagaacaataaaatcaaatatctgtctagttgctttgggttaccaccaaggaccaaatttgcccattg
cccataaattggcctattttatgcaaacatatgcaatggtttttggcctatgggctgttacccagaactgggccccttgctgttgaaagggaataaataa
taaatccttgaaatagaaataaaaaggaagtgtggagtgaatctgggtcctgtccacaaaaaggttaagaaataccatctaatggctagttaatttgcat
ttaacaagaaagtaatctcatgataagaaacaattttctgccaaaaggtgccatagattgcttaagcattacaatctctttttctagatgaggtgcgcac
tggcacataccgccaactcttccacccagagcaacttatcactgggaaagaagatgctgccaataactatgcccgtgggtcactacacaattggccaggn
agatcatttgacctggtgcttggacaggatcccactc
3' EST: CF785771
[+]gtccagcaccaggtcaatgatctccttgccaattgtgtagtgaccacgggcatagttattggcagcatcttcctttccagtgataagttgctctgggtgg
aagagttggcggtatgtgccagtgcgcacctcatctagaaaaagagattgtaatgcttaagcaatctatggcaccttttggcagaaaattgtttcttatc
atgagattactttcttgttaaatgcaaattaactagccattagatggtatttcttaacctttttgtggacaggacccagattcactccacacttcctttt
tatttctatttcaaggatttattatttattccctttcaacagcaaggggcccagttctgggtaacagcccataggccaaaaaccattgcatatgtttgca
taaaataggccaatttatgggcaatgggcaaatttggtccttggtggtaacccaaagcaactagacagatatttgattttattgttctacctgtatctgg
ttgaacaatgctaatcactaattggttgctgtgagtttttacccaggtgcaaatttgcccagtgttggtaaatgaacttcagggttacaaataactcctc
aataaatcataagctgcttagtaataaatcanatcccanatacacagaaaggaatatcatttaacctangtaattataattcacatttcagaaagactgg
gggggggggggggggaattaatttaactccgagttgccaaaaaggaacaagtaaccttggaatttcgggccccggatagaaacccaaggtggcagttggg
accttggggagtggaaaccctgtgggagaagaaaaggccaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: