XB-CLONE-4350047
Clone Name: IMAGE:7025745
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Gene:
gamt
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785773
[+]
taattaatccccccccccccctctctctctcccctgtctactttgaacaaccaaatcaatgtctgaacggatcttcatagagggcgagagctgcaagtcc
gcctggcacaatgcaactgctgggtacgatgataccgacactcacctggagatcctgggtaaaccggtaatggagcgatgggagactccatatatgcaca
gtctggcttcggtagcggccagcaagggtggccgtgtgctggagattggttttggcatggcaattgcagctactaagttggaagagtacaatatcgagga
acactggataattgaatgcaatgatggtgtattcaagcggcttcaagaatgggccacaaagcaaccacataagattgtgccattaaaagggctgtgggag
gatgtagtgccaacattacccgatggacactttgatgggatcctttatgacacataccctctttccgaagaaacatggcacacgcaccagtttaacttta
tcaagggtcacgcttgtcggctgcttaaacctggaggagtcctcacctactgcaacctgacatcttggggtgaactactcaaggccaaatacaatgacat
agaaagaatgttccaggagactcagattccacaactagtggatgctggttttaagtctgagaatattagtactactgtaatggacctgataccccccgag
gaatgcagatactactccttcaaaaaatgatcacaccaaccgtttatttaagtctgagaaaactttgaaaacctaccttggttaataaggaaccttagac
ccct
3' EST: CF785772
[+]tttttttttttttttcaatatatatggttttattaagcatttgctagcagagtgcttgctgtataaaatggaactttgcaactctgccctgatggcctta
aacagtctatcatattgtggtaggtccagataaatccagtaattattttgtagatagccagtggtgacaattttgattcctgtccctgttagggtctagg
ttcctattaacaaggtaggttctcaagtttctcagaccttaataacggttggtgtgatcatttttttgaaggagtagtatctgcaatcctcggggggtat
caggtccattacagtagtactaatattctcagacttaaaaccagcatccactagttgtggaatctgagtctcctggaacattctttctatgtcattgtat
ttggccttgagtagttcaccccaagatgtcaggttgcagtaggtgaggactcctccaggtttaagcagccgacaagcgtgacccttgataaagttaaact
ggtgcgtgtgccatgtttcttcggaaagagggtatgtgtcataaaggatcccatcaaagtgtccatcgggtaatgttggcactacatcctcccacagccc
ttttaatggcacaatcttatgtggttgctttgtggcccattcttgaagccgcttgaatacaccatcattgcattcaattatccagtgttcctcgatattg
tactctttcaacttaatacctgcaattggcatgccaaaaccaatctccagcacacgggccacccttgctgggccgctacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: