XB-CLONE-4350031
Clone Name: IMAGE:7025739
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Gene:
hnrnpdl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785783 [+]
taattaatcccccccccccccgcttttccacatttgcttagtgtgtgtgttcgtaaggggcttttgtattcgtcaattagaccgtaatcatgaccggatt
tggtgccaccccggacttcaacgaaggctcaaaaatcaatgccagcaaaaaccagcaggatgaaggcaaaatgttcatcgggggtctgagttgggataca
agtaagaaagaccttacagagtatctgtctcggtttggggaggtggtagattgcacaatcaaaactgatccagtgactgggcgttcaagaggatttggct
ttgttcttttcaaagatgctgtgagcgttgacaaagtgcttgaaacaaaagagcataaactagatggcaagctcattgacccaaaaagagctaaggcact
aaaaggcaaagagcctcccaagaaagtgtttgttggcggactcagtcctgaaacaacagaggagcagatcaagcagtattttggtggatttggagaaatt
gaaaatattgagctgccaatggacacaaaaacaaatgaaaggagaggcttttgttttgtcacctatacaggcgaagaaccagtaaagaaacttttggaaa
gccggttccatcaaattggtacaggaaagtgtgaaataaaagttgcacagcccaaagaagtctacaggcagcaacaacagaagcagcaaaaaggaggaaa
gaggagcaacccactggaagggngtggtgcccaaaggaaagggggcagaggttcaaggtttggaattcaaagcctacagccaacctaactaatggattca
gaaactaatgggaaagcctt
3' EST: CF785782 [+]tttttttttttttttagctgttcttacttttattacaaacttcatttacatcaaaattgaatgcaccgatcacttcacaagcacaaacaaggaacttaaa
aatattgctgaacataaaacctctttcatacagatggcaaaaaaatgccattgaaataaaaaaaaaaaaaaaatcccagcttctatttacagcaagacac
ttagtgcctcagtcatagtcctctcaaaagagtaacatgatggcggctcccggcattgtcgcaggatgttaagtctctttatccattgccaccttaggtt
tcaatagtcaccgctttagtaaggctggtaattgttttgatggtttcctccacctcttgctttgccatatgtgctttgttgcccactgtagtctgtgtat
ccttggttatatccatagcttccatagttatatccagagtaatcataaccactataactgttattatacccttgatcagcataagaaccattgtttccat
agcttccatagttctgatcatagtagttgctgtagccttgattccaaccttgacctctgccccttcctctgggaccacccccttccagtggctgctcctc
ttcctcccttctgctgcttctggtgttgctgcctgaaaactttctttgggctgtgcaacttttatttcacacttttcctgtaaccaatttgatggaaccc
ggcttttccaaaagtttcttttaccgggttcttccccctgtaaaggtggaacaaaccaaaacccccccccctttaatttggtttttgggggccactgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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