XB-CLONE-4350015
Clone Name: IMAGE:7025732
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Gene:
acta4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785792
[+]
taattaatcccccccccccccgagcgcacagctactcctactctctccctgctgcaaaagtcacagtccctctccagactcaaacatgtgtgacgacgat
gagaccaccgctttggtgtgcgataacggttctgggctagtgaaagctggctttgccggagatgatgctccccgtgctgtcttcccctcaattgtgggac
gtcctcgtcaccagggtgtcatggttggtatgggccaaaaggactcctatgttggggatgaagcccagagcaagaggggtattctgaccctcaagtaccc
aattgagcatggtatcatcaccaactgggatgacatggaaaagatctggcatcacaccttttacaatgagttgcgtgtggccccagaggaacaccccacc
ctgctcacagaggcacctcttaaccccaaagccaaccgtgaaaaaatgacacagatcatgtttgagaccttcaatgttccagcaatgtatgtagccatcc
aggccgttctgtccttatacgcatctggacgtaccactggtatcgtcttggactctggtgatggtgtgacccacaacgtgccaatttatgaaggttatgc
tttgccccatgccatccagcgtctggaccttgccggacgtgacctgactgactacctcatgaagatcctgactgagagaggctactcttttgtcacaaca
gcctgaaaggggagattgtggagggacattaaagaggaagcctgccctatgtgggctcctggacttttgaaaaatgaagatggcgaacaggcagcccacc
ttccctcaatcttttgggaggaaagaacct
3' EST: CF785793
[+]tttttttttttttttcatgtttataacaaatgttttattgtaattttgtatatatttgtgacatttgtacacagaggaattggggtaagaatataattag
aagcactttctgtgcacaattgaggggccggcttcatcatattcctgcttgctgatccacatctgttggaaggtggagagagaggcaaggatggaacctc
caatccatacagagtatttacgttctggaggagcaataatcttgatcttcattgtgcttggagcaagggctgtgatttccttctgcatacggtcagcaat
accagggtacatggtggtaccaccagacaaaacattgttggcatagaggtccttacggatgtcaatgtcgcacttcatgatggagttgtaagtggtctca
tggatacctgcagactccataccaatgaaggatggctggaagagtgtttctgggcaacggaacctttcatttccaatggtaatgacctgtccgtctggga
gttcatagctcttctccaaagatgaggaagtggctgctgttgccatctcattttcaaagtccagagccacataggcaagcttctccttaatgtccctcac
aatctccctttcagctgttgtgacaaaagagtagcctctctcagtcaggatcttcatgaggtagtcagtcaggtcacgtccggcaaggtccagacgctgg
atggcatggggcaaagcataaccttcataattgngcacgtttgtgggtcacaccatcaccagagtccaagacgatacccagtggtacgtccaagatgccg
tattaaggacagaacggcctgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: