XB-CLONE-4350000
Clone Name: IMAGE:7025726
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785801
[+]
taattaatcccccccccccccgatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagagaa
aggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacagaga
cggcatcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcatct
ggccccatcaacttcaccgtcttcctgaccatgttcggccagaagctcaagggtgctgatcccgaagacgttatcactggggcatttaaggtgcttgatc
ctgaggccaaaggatccatcaagaaacagttccttgaggaaccccttaccaaacagtgtgacaatattcccccccctaggaaattaaaaacatgtgggac
agcctttccccccttgattgttgcctggtatacgtaggactaacaaagaaccaccccgcaaatgttccttcccccccacctggagaaggaacaaaacgac
ccccgggaattttatttagggccccccgggtcctttgggcccctctcctcttagaataggatagtgggggagttgctcgccactattgaatctgtcctct
atgcccacccaccagcccacttcgcgctaggggaaagcgttcggcggggacatgtcaatttatttgagacaaaaatcctctgaaattatccctattctcc
gcgcggagacaatataattcttgcgtccaatatcaccctatgtctatattgtatcctaaccaagtgggtgagtggttcctgctcaaatacgggggagtat
tagctcccccgccttacacctacccgacatgttctacctgtacgcacaccttggtcttcctctcccgcgtgngtgtacacactctcttctcactttctgt
atatattatgttaacacgccccctctatattagaagcataaatttaaagtgcagcacccaccaatagagcattg
3' EST: CF785802
[+]tttttttttttttttctgaataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagggag
ggtaaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttgtgt
ctctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactgggcct
gtttattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccacatgt
tcttgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcaggatcaagcacctt
gaatgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtcaggaagacggtgaagttgatggggccagatgcctctttg
accatctcttccaactcttcattcttcacgttcaggcggcccatagctgcgaatgtgtccctcaggtcttccttgtcaatgatgccgtctctgttctgat
caatcactgtgaaagccctcttgaaactctggatctgggtttggtcaaacatggacaacacattgggaagatcaccttctgctgcctttctcttgggctt
tcttgctggtgccatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: