XB-CLONE-4349995
Clone Name: IMAGE:7025724
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Gene:
tnni2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084508 [+]
GCAGTCCCACCCCTGCTTCATAGAGAGGGCTCAGTCTACGAGGGTCAGGTATCACAGAAACCGCCAAGATGGGTGAAGATGACAAGAAATCCAAGATCTC
CAATACTCGCAAAACCCACCTGAAGAGCTTGATGCTCCAGGTGGCCAAAACTGCTCTTGAGAAAGAAGAAGCTGAAATTGCGGCAGAGAAGGAAGCCTAC
CTACAAGAGCACTGCCCACCTCTAAGCCTTCCCAGCAACTTATCAGAACTCCAGGATTTCTGCAAGAAGATGCACGCCAAAATCGATGTGGTTGATGAGG
AGAGGTATGACATGGAAGTCAAAGTTGGAAAGAGCACCAAGGAGCTGGAAGACTTAAACCAGAAGATCTTTGATCTGCGTGGCAAGTTCAAGAGGCCTGC
CCTTAAACGTGTCCGTATGTCAGCTGATGCTATGCTGCGTGCTCTGCTTGGCTCCAAGCACAAGGTCAACATGGAACTCCGTGCCAATCTCAAACAAGTC
AAGAAGGAAGACACTGACAAGGAGAAGGATCTCCGTGATGTTGGTGACTGGCGTAAGAACATTGAGGAGAAATCCGGCATGGAAGGCAGGAAGAAGATGT
TTGAATCTGAGGCTTAAGCCCTTAACCAAGACCACTGCCATCTTAAACCCCTTAGCTTCCAGAGACAACCACCATACCCTAACTTCTCTGTTAACTAGAG
GGTTATTTCCTCTGCTGCTCACTCTCCAGCGGCAAAGGGGATACTGAAGTCTTACCTCCTCCCCACTCCTGGATATTTCACCACTCCACTTTATACCCTT
GTAGAAGGTCTTAAAGAATGTTATGGACTCAAGGAGGGGCTCAAATTTCTGGGTCCCCTCTGTATTGGCTTTGCAGCAGTTGTGTTATGCGGACGTGAAA
ACATAAACTTGTAAATAAAGCTTGTGACGTCCCTTAAAGTTGGTGATACCTGATCTGGAGGTGACGTCACGGTCCTTTCTTCTCAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAA
AA
5' EST: CF785804 [+]taattaatcccccccccccccgcagtcccacccctgcttcatagagagggctcagtctacgagggtcaggtatcacagaaaccgccaagatgggtgaaga
tgacaagaaatccaagatctccaatactcgcaaaacccacctgaagagcttgatgctccaggtggccaaaactgctcttgagaaagaagaagctgaaatt
gcggcagagaaggaagcctacctacaagagcactgcccacctctaagccttcccagcaacttatcagaactccaggatttctgcaagaagatgcacgcca
aaatcgatgtggttgatgaggagaggtatgacatggaagtcaaagttggaaagagcaccaaggagctggaagacttaaaccagaagatctttgatctgcg
tggcaagttcaagaggcctgcccttaaacgtgtccgtatgtcagctgatgctatgctgcgtgctctgcttggctccaagcacaaggtcaacatggaactc
cgtgccaatctcaaacaagtcaagaaggaagacactgacaaggagaaggatctccgtgatgttggtgactggcgtaagaacattgaggagaaatccggca
tggaaggcaggaagaagatgtttgaatctgaggcttaagcccttaaccaagaccactgccatcttaaaccccttagcttccagagacaaccaccataccc
taacttctctgttaactagaggggtatttcctctgctgctcactctccagcggcaaaaggggaatactgaagtcttacnctctcccccactccctggata
tn
3' EST: CF785805 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
tttttttttttttttttaaaaaaaaaagggggggggccccccccccaaaggggggcccccccttttggggggggccccccccttttttttaaaatttttt
tttttccccccccaaaaaaaacggggggggaaaccaaaaaagggggggccccaaaatttggcccccccccgggggcccaaaaattttttaaaaccccccc
caggggggaaaaggggggggggggaaaacccccgggggggggggggggaaaaaattttttccccccttgcccggggggggggggcccccaaaaaaaaacc
cccctttttaaaaaaaaaaatggggggggggggggttcccccggaacaaagggggttaaaaaggggggggtctttttttaggggtttacccccaaaaaaa
aaaaatttttttccggcccccggggggggatttccccccgggggtttaccccccccccccaaattggggggagcccccccccggggggggggcccccctt
taaaattttaaaaaaagggcccaagttccctgttgcccctgggggggggggcccccaaaaaacccccccaaaaacccccgcggaaaaaagaaactttttg
ggggggggcccttttaaattccccccccaaaaaaaaaaaacctgggggaaaaaaaccccccccccccccgggggggctttcccccaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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