XB-CLONE-4349986
Clone Name: IMAGE:7025721
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Gene:
zp3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785811 [+]
taattaatcccccccccccccattgtgctgttcctgctggaatcagaatgagacagttggggagactgaatgtcctactgttggtcctgctgggacctgt
ctatgctttggcccattctgtgtcctctgagaggagtcggcggcaggggagccaatggtggcaagactaccattctgttgtatctaactatggccccagt
agggggactaacggcctgggcttcggctctaggcagctccccggctccccccagaacacggtcagtgtgcgctgcaatgaggacaagatggtggtgacgg
tgcagacggatctctatgggattgggaagaacgtcaaggcctccgacttgactctgggaccccagcgatgcccccccagtgccccccagagcacaagcac
tgccgttgtcttccaggttaacctgcaggactgtgggaacagcttacagatgactcccaacttcctggtctatagcaccaacctgacctacgctcctgcc
cccaggaatgttcccattatccggatgaatggtgccaaggttctcattcagtgcttctatcccaggaatggaaatgtcagtagtaaagccatcaaaccaa
catgggtgccatttagctccaccatatctgctgaagatagactagcattctccttaccccttttgaccgaagactggtctgctccaaggccttcttatgt
ctttcagttggggggacgtctttccacataaaaagcttccatcaacatttgctcaaccatggccccttataactttttaatttttgacccttttgtggtg
ggccaccaatttgcccccctgaatc
3' EST: CF785810 [+]tttttttttttttttgttgtctttatttctggatagagatacatattgcttccttttcatgagcttagtggccacaacagcaacacaaccagccagcaca
acaagccccaaagaacccaacgccagaagctcccacagttccaaggtcttgctctccggttcctgctttagagcttgtgtttgagtgtattcaggggtga
ccaccaatatgggccctagagtggccaagctatgttcttcctcctccatcttagatacatgcacggcttcccgtttcctgttccacctagagcctgaaaa
gtatggtcccagtcttcggctttgggatggtaccgacacacagttccccgcatcacagcagctgcatatgttactgggtccttgaagaggagaccaactg
tttgcggatttgctgaaggagcaagccttgttcatggtgtctgggacttgggtggcagcagcagctctcagattgcaagtgatgtaaatcacagaggcat
cggttgtagtaaacctgaaggcatcaacagagaactgaagcttgtcaggctgggtccttggagatcggaaggcagaaaaggagtcctcttgttttcccat
ctaccaaaaaacccattggtgattaaattatcccaaaaacgagggtttggaaatttaacattcagggcgcaaaatgggtggccccacaaccggttcaaca
atattaataggtcataaggttggctgggttgggccaaagtttgaagggaaaccttcctaggtggaaaaaacttctcccaaactgggaaggaaatttagaa
acgccctttggagccagaccaaatacatcgggacgat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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