XB-CLONE-4349975
Clone Name: IMAGE:7025713
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Gene:
rpl17
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077000
[+]
CCGCTCTTCCTGCGCGGCCTGAGATTCTCTCGGAACAATGGTGCGCTATTCTCTCGACCCGGAGAACCCCACAAAATCATGCAAGGCACGGGGCTCAAAC
CTTCGAGTGCACTTTAAGAACACTCGTGAAACTGCTCAAGCTATCAAAGGCATGCACATCCGTAAGGCCACCAAATACCTGAAAGACGTCACCCTGAAGA
AGCAGTGCGTTCCTTTCCGCCGCTACAACGGGGGTGTTGGAAGGTGTGCCCAGGCTAAGCAGTGGGACTGGACACAGGGACGTTGGCCAAAAAAGAGCGC
AAACTTCCTCCTGCACATACTGAAAAATGCTGAAAGCAATGCAGAAGTCAAGGGTTTGGATGTTGATTCTTTGGTCATTGAGCACATCCAGGTGAATAAG
GCCCCCAAGATGCGCAGGCGTACCTACCGTGCTCATGGTCGTATCAATCCCTATATGAGCTCCCCATGCCACATCGAATTGATCCTTACTGAGAAGGAAC
AGATCGTACCAAAACCTGAGGAGGAAGTCGCTCAAAAGAAAAAGATCTCTCAGAAGAAGCTGAAAAAGCAGAAACTCATGGCTCGGGAATAAATACAACC
TGTACAGAAATAAAGTCCCAAAAAACTGTTAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF785818
[+]taattaatcccccccccccccccgctcttcctgcgcggcctgagattctctcggaacaatggtgcgctattctctcgacccggagaaccccacaaaatca
tgcaaggcacggggctcaaaccttcgagtgcactttaagaacactcgtgaaactgctcaagctatcaaaggcatgcacatccgtaaggccaccaaatacc
tgaaagacgtcaccctgaagaagcagtgcgttcctttccgccgctacaacgggggtgttggaaggtgtgcccaggctaagcagtgggactggacacaggg
acgttggccaaaaaagagcgcaaacttcctcctgcacatactgaaaaatgctgaaagcaatgcagaagtcaagggtttggatgttgattctttggtcatt
gagcacatccaggtgaataaggcccccaaaatgcgcaggcgtacctaccgtgctcatggtcgtatcaatccctatatgagctccccatgccacatcgaat
tgatccctactgaaaaggaacagaatcgtaccataacctgaggagggaagtctctcaaaaaaaaaaaaatctcttcagaaaaaagcctgataaaagtcga
aaacctctgggcctcggggaaaaaaatactatccggtaccagaaaataaaagttcccaaatataactggttttaattttgattcacgttctctctcttta
ggggtcccccttattttcttataaagtcaggcccccccctagcgccgtttggagaaagtcttggcccccagagggccccaatacttttgttttttatttg
cactgaacctttattaaattggcgttttccccaatataataagaggataaatgaacgcaaatgccacacaaaatatttttgcaccaagtagaaaata
3' EST: CF785817
[+]tttttttttttttttcttttttaacagtttttntgggactttatttctgtacaggttgtatttattcccgagccatgagtttctgctttttcagcttctt
ctgagagatctttttcttttgagcgacttcctcctcaggttttggtacgatctgttccttctcagtaaggatcaattcgatgtggcatggggagctcata
tagggattgatacgaccatgagcacggtaggtacgcctgcgcatcttgggggccttattcacctggatgtgctcaatgaccaaagaatcaacatccaaac
ccttgacttctgcattgctttcagcatttttcagtatgtgcaggaggaagtttgcgctcttttttggccaacgtccctgtgtccagtcccactgcttagc
ctgggcacaccttccaacacccccgttgtagcggcggaaaggaacgcactgcttcttcagggtgacgtctttcaggtatttggtggccttacggatgtgc
atgcctttgatagcttgagcagtttcacgagtgttcttaaagtgcactcgaaggtttgagccccgtgccttgcatgattttgtggggttctccgggtcga
gagaatagcgcaccattgttccgagagaatctcaggccgcgcangaaaaacgggggggggggggggggaaatatttactccgagggca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: