XB-CLONE-4349970
Clone Name: IMAGE:7025711
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Gene:
eef1g
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785821 [+]
taattaatccccccccccccgccctttctactcgcgtgaagatggccggcgggactctgtacacataccctgataactggagggcatacaagcccctcat
tgctgctcagtattcagggttccccatcaaggttgcctcctctcctccagaattccagtttggagtaacgaataagacatccgagttcctaaagaaattc
cccttaggcaaggtaccagcatttgagggcaacgacggtttttgcctttttgagagcagcgccattgctcattatgtgggcaatgatgagctccgaggaa
ccaatcgtttacaccaagctcaggtcatccagtgggtcagcttctcagacagtcacattgtccctccggccaacgcatggggcttttcccactctggggg
tcatgcaatacaataagcaaggccccaaaaaaacccaggaggagaaaaaaggggtgcgcgggggtgttttaatattccccccaagaaaaaccgcggtttc
ttccttggtgggcaaaagaggcccacggctgaataaaaacaataaatttactttgcctccccttgctgcgggactcaaataaaacagggtgactgaaaaa
cccttccttttcccccctgtccaattttggaaaaaagctaaaaagggtggggcttggtgaaacctgaggtttaaatctaccccgaaacttctcctggctt
gtggattggggaagaaaatcaaattcttttgaggaacaaagaatgggcaccgtttttggaatgtcataaataatttttcccgaaaaatgcagccccaagg
aaaaaaaaacctctcaatgaaagaagaaacccatctaaaagaatcctcaaattatgaatagggaggcaatta
3' EST: CF785820 [+]tttttttttttttttggtttcagtttttttattttcggtttgctgtcttgttaagttaaaaggctctggatgttggtacggatgtgaaaaccagactctg
cgcgtattttagggggacagagatcgcctcctcctcatttgaagatcttgccctggttaaaggcctttcccacgtgcttgaagtctccctcccagcagaa
gtattctttgaccagggttttgcactcctcactattgctgtccagctttctccaggtgtaggattcatagtcaatttgccagtcttcagagagtgtgaat
gcaaggtcctggcccctgaacacccacacgccggagatggagctgtcattgttggtgccaaacaagatgacgctggcaaagccagtcttgcgcagtttgt
ctagacgctggaacatacctgtaattaagttgcagctcatgaaggtttgtgtcagctcctcggggaatttgtattcagcgtaccagatggaccagccttc
cttgtcaaagtgctcccagaaataaggcagagctacagccagggtatcttcattagagtattttctcttaaactcatccattacaaaagagcttttaggt
agatgtgcgtatgggtcctttgatttaggctctgctgctaaagccttttcagattcatccatatcatcctcangggctgggggcagagcaggagctggtg
ttgcccttcttcctttcctcccttttcctttttttggctcctttgctggcttctcctttcttgggagtttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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