XB-CLONE-4349961
Clone Name: IMAGE:7025708
|
Gene:
aldoa
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785826 [+]
taattaatcccccccccccccgagagctgagacctcccctgagcaccctgcccgcttcctacagcacccgctctcagatagtagcaccatgcctcaccag
tacccagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagccgatgagtccacag
gcagcatcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccgacgacagagtgaa
cccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtc
gtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgagcgctgcgcccagt
acaagaaagatggcgctgacttttgcaagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgagaatgccaacgtatt
ggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagccccgagttctccctgatggagacatgacctgaagaggtgccagtatg
tgacagagaaagtcctggctgcagtgtaccaggctcttagcgacccacacatctacctggaagggaccctgctgaaacaaaacatggttacccccgggca
tgcctgcc
3' EST: CF785827 [+]tttttttttttttttgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggt
gcacgtagcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagt
ccgcagtctctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagc
ccctcactaggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagct
cccgcctctccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttct
tgcccccccaggctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcaggnggcactggttgatggcattgag
gtgcacggtggcctcttcctcactctgacctccggacaggaaggtgattcctcaacagcagggggcactgtgcgccctgaggcggtgactgttggcatag
caacctcctgaaggggggtacttcntgggtgcagggatgccccggggggtaaccatgtttgggtttcaacagggtccccttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: