XB-CLONE-4349932
Clone Name: IMAGE:7028310
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Gene:
strada
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081364 [+]
CGCCCTTCCGAGGAAAGGAACTAGCCGCTACCGTAAATAAAGCCTCCACTACACATCCAGACTGTACAGTTTATGGCCTTTGAACGCCCGGGCAGCACTA
AAAGGACCCCATGTCTTTTCTTCGCTGGGTGTCAGACAAGATCCTGGTTGATGGTTTGAGAGAGCTGGAGTTTTTTGGAGACCAATGAAGCAGCGAGTGT
TGAATCCGTAGCATCCTCCCATGGTCCCAACACTATGGGGATCTACACCCCGGACAGCAGTTGTTATGAACTGCTTACTGTGATAGGTAGAGGATTTGAC
GACTTGATGACTGTAAATTTAGCTCGTTACAGGCCAAATGCAGAATATGTATGTATCAGGAGGGTGAATTTGGAGTTCTGCACCAATGACATGGTTACCT
TCCTGCAGACTGAGCTCCACGTGTCCAAGTTGTTCAACCACCCCAATATACTTCCCTACAGAGCCACATTTATAACTGACAATGAGCTTTGGGTGGTTAC
TCCCTTTATGGCTTATGGTTCAGCCAAAGATCTAATCTGTACTCACTTTACAGATGGGTTGAGTGAGCTGGCGATCGCATACATTCTTGTAGGAGTTCTA
AAAGCTTTGAATTATATCCATCATATGGGATACGTTCATAGGAGTGTGAAGGCAAGTCACATCCTCATTTCTCTGGATGGCAAGGTTTACCTCTCTGGCC
TACGCAGCATCCACAGTATGATAAACCATGGGCAGCGCCTTAAGATGATACATGACTTTCCCAAGCACAGTGCCAGGGTCCTTCCCTGGCTGAGCCCAGA
GGTCCTCCAGCAGAACTTACAGGGGTATGACGCTAAATCAGATATCTACAGTGTGGGAATCACAGCATGTGAGCTTGCCAATGGCCATGTACCGTTTAAA
GATATGCCAGCCACTCAGATGCTGCTGGAAAAGTTGAACGGCACAGTCCCCTGCCTGCTGGACACAGCCACCATTCCAGCCTCCGAGCTTACTATGAAAA
CATCTCGTTCTGGAGCAGACTCTGGCATTGGAGAGGGGACGTCCAGTCGTCCTTCCAATGGGGAGCCATCTATGCACCCTTATAACCGCAGCTTTTCGCC
GCAATTCCACAGTTTGGTGGAGCAGTGTCTACAGAGAAACCCTGAGCTGAGGCCGAGTGCAGGTACCCTCCTCAATCATTCCTTCTTCAAACAGATAAAA
CGCAGAGCATGTGATGCCCTCCCAGAACTGCTTTGTCCTGTCTCGCCAATCACCAGCTTTGAATCAGCAAGAACACTGAATCCCAGTCGGTTTTGGAAGT
GGCATCTGGGTTGGAGCAACTAGCTGTAGAGGACTGGGACTTTTAGGGTAAAAAGGAGGAATTCAGATCCTGTTCATGTTTACACTGCACGTATCCCAAG
CGGTTCATTGGGAATGAGTGCAAGGGGGGGTTAACCCTTTCACTTACACTGCTTAGGAGTTTGTACAGCACCATTGTTTATGGTGAAAACTCTTCAGCAC
TAGAACTCACGTCTTTGTACATTTCTTTCCCATTGCCTGTGCTTGATGACTGGTGTATTTAATTCCCCTGTTTTTGTATGCTGAAACTGTTTGTTCAACA
GCAGCCCTCACTCAACACAATTTGTTTATTTTCTGGAATCTGGATGTTTGCGTCAGAACCTCTCATGCTGGTTATGTGTTAATAATAAGGCAATTTATTT
GCAACGTTGCATGTTTCTCTCCAGCTGTTCCTAAACAGGGATCAGGGCCAGAGATAAAGAGCAATGCAGTAAGGCCACCAGCTAAGCATATTTTTAGACC
AGTGGGGCCTAAGGCATATGCTGTCTCTGCCCTAAACCTGGTTCTGATTGGAAGCTGGAGTTCTGTGAATTAAACAGAAGTTACCATTCATTTATTGATA
TGCTTGGGGTCTCTGGGCAAAGCAGACCATCCAAGTGCTTGACTTGAAAACCAGTGACTTACCTTATGTTTTTTTCAGGCTATCACGTCTGACTAGTTGA
TAATAAGTTTAGAATTGACTATTCAAATTTGCATCAACTTTGTATTTTAATATACCTGATTTCCATTCATATTTTAAACTGACATGTGATGTTCACATTG
CATGAAAGTATGACACTTTATAAAGCTGTGTTGCCCTTTTGTCTCTCATTTGATCCCATTTTTCTTTCCCAGCTGAGCCTTCTGTTGCCCACTTCAATAC
CTTCCTGCCCCGTCCACTTTTTAAAACCCTCTCCAACGTGGTGCTGCTGCTCATGCAACCTTATGCAGTGCTCCTTCTGCCCTGGATAGTGATGTCATAT
GTGGATAAAGTAGGTTAATTGGATATTTGAACTAACATTATGGGTGCATATGATGGCAGCAGAAATGGTAAGCCCATGATTGAAAATAGAGCACCATGTC
TAGTCTCCAATGGAAAAAGAAGAGATTGCAGTCTATTCCTGTCTTAGGTAAATCTCCCTTCCCTGTAGAGTGAGGTGTCTAGCTGGCAGCAGCACTGACA
GGTCAGTCTTATGTTATAAGCTGGAGATACTCTGTTACACACATTTTGTTGCCAAAGGAATAAATTTTAAATATTTCACTACAAAAAAAAAAAAAAAAA
5' EST: CF786125 [+]taattaatccccccccccccccgcccttccgaggaaaggaactagccgctaccgtaaataaagcctccactacacatccagactgtacagtttatggcct
ttgaacgcccgggcagcactaaaaggaccccatgtcttttcttcgctgggtgtcagacaagatcctggttgatggtttgagagagctggagttttttgga
gaccaatgaagcagcgagtgttgaatccgtagcatcctcccatggtcccaacactatggggatctacaccccggacagcagttgttatgaactgcttact
gtgataggtagaggatttgacgacttgatgactgtaaatttagctcgttacaggccaaatgcagaatatgtatgtatcaggagggtgaatttggagttct
gcaccaatgacatggttaccttcctgcagactgagctccacgtgtccaagttgttcaaccaccccaatatacttccctacagagccacatttataactga
caatgagctttgggtggttactccctttatggcttatggttcagccaaagatctaatctgtactcactttacagatgggttgagtgagctggcgatcgca
tacattcttgtaggagttctaaaagctttgaattatatccatcatatgggatacgttcataggagtgtgaaggcaagtcacatcctcatttctcttggat
ggaaaggtttaccttctctggccctaccgcacatcccacattaagaataaacccggggccaggcgcccttaaaatggaaaaatgacattttcccaaagca
caagtggccacggggtccctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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