XB-CLONE-4349886
Clone Name: IMAGE:7028164
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Gene:
slc30a8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084148 [+]
CGAGAAGTCACCAAGAGCTGATGAACTTCTGCTTCATTATGATTTGTGTCTCTTTCATGGGCAAAAGGTATTCTGAGTAACACCCCGACATCCTAAGGGG
ACCCTATTTTTTTCCTGTAATCGTTATGAAGGGATCAGAAGAAGCCTACCTGGTGAGCGACAAAGCAACCAAGATGTACTCCTTAACTAAGGACAGTGAA
AAGAATCACCCCAGCAAACCTCCCCTGCAGGACGAAGAAAACCCACAGAGCAAATATCACTGCCACAATAATAACAAAAAGGCCTACGATGCCAGACAAA
GAGAACAAACGTTTGCCAAAAAGAAACTGTGTATTGCATCACTTATATGCTTCGTATTTATATCTGCAGAGATTGTAGGTGGGTATATCGCAGGAAGCCT
CGCTGTTGTAACCGACGCAGCCCATCTCTTAGTAGATTTGTCTAGTTTCTTTATTAGTCTCTGTTCCCTCTGGCTGTCATCAAAGTCTTCAACGACGCGG
CTAACATTTGGATGGCATCGAGCGGAAATTCTGGGGGCACTTATGTCCGTTATTACAATCTGGCTGGTGACTGGAGTGCTGGTGTATTTGGCCTGCGAGC
GATTAATTCGGCCTGACTATACCATCGATGGGACCGTAATGCTGATAACGTCCGCCTGTGCCTTAGGGGCTAATTTAGTCTTGGCTTTGATTTTGCACCA
ATCAGGACACGGACACAGCCACGCAGGAGGCAAACACGAGCATATGGCATCGGAATACAAGCCCCAAACCAATGCGAGTATACGCGCAGCCTTTATACAC
GTCATTGGGGATCTGTTTCAGAGCATTAGTGTTCTCATTAGTGCACTGATTATTTACTTTAAGCCAGAGTACAAGATGGCTGATCCCATATGCACCTTTA
TATTCTCCATTTTTGTGTTGATCACAACTGTAACGGTGCTAAGGGATCTCTTGACTGTCCTAATGGAAGGGACTCCGCGAGGGATTCATTACAGTGACGT
GAAGCAGTCCATTCTGGCTGTAGATGGGGTTAAATCAGTACACAGTCTGCATCTATGGGCTCTAACCATGAATCAAGTCATCCTTTCAGCTCACATTGCC
ACAGACATTGTTGGGGAATCAAAGAGAATTCTGAAGGACGTCACACAGAATGTATTCGCCAGATTCCCTTTTCACTCAGTCACCATTCAAGTGGAACCAA
TAGAAGACCAGAGTCCGGAGTGTATGTTCTGCTATGAGCCAACACAGTAATTAAAGTAATCGCCATCCATTGGGCCTCATTACAGCCAGCCAATGACTAT
TTTTCCTAAAGGTTAATTTACAAGGAAACTCTACCACTCTCAGCACCACCAGCCCAAGGTGGGCAAACAATTGAACAGATGTTAGGGCGCACAATGTCTA
AACTGCATGTCTAGATTATAATTTCTGGGTGTAGCAGTAGCACAATAAAATATTAAGACGTATTGACCCCCTTAAACAGCCTCCTGTTTATACAAGGCCC
CGGGAAAATAAACTAGGGTGGTTATTTTATTCCCATGTGAAATTTCTGCCCTTCAGTGAAGAGCTGACAGATACATGCGCTTCCCGATCTGCTTCGGTGC
CCTCAGCGCCATACGGAACTCTGCAGAGAAGAACACATGAATCTGATGTAAAGGAAGCAAGATAGCTTTATTTAGACTGGCCAATTAAAATGGAAAAATG
GACAAGTGACCAATATGCTATGATTGCTTAGCTGCCTCTCATGCTAATGTGAAAAAATAATAATTATCATTATAATACATTTACTATTGTACAAATGCTT
TAATGGACTATTAAGTATACCATATAGTATACCATATACCTATTGCATGCCACCAATTACCGTTGCCATCTTTAAATCCCATCAATCCCCCCCACCACCA
CTCATATAACCATCTACTCCTCAGTGCTGACTTTATATACTCTCCTGTATAGCCCCTCAAAATCCTCTTCTTTCTCCATATATCATATCAGCTTGACTCT
TCTCACTTGAGATCTCCTTATTCGATTATCTCGTATGATATCATATGTCCAACTTGTAGAGTACCCCCTGCACCTACACCCAGGCATGGATTTGTGGCGA
GGTCCCAAGGGCCTGGGCCTAGCACGGCACAATTATAGGGGCAACATAAGGAGCAATGGGTACCGGACTTGCAGAAAACTTCAGCGTATCCTCTCCCTAC
TGCTCCGTAAGTGGGGGGGGGGTGGCCTCGGGGTGCCGTATTAACAAATCCTGCCCTTCCCACACCCAATAACTTTAGATTGTTGAGAATAAAACAGAGT
TATAGCCTTGACCCAAGTGCCATAGACGTTAATGCCCACTTGTTTGTTGAGGTTTGTTTCGCTCTAGGGCCATGGGAGCCGTCAGACTGAGAACTGCATC
AATGAGCCGATGCTGTCCCCAATCCTGATTCTAGGGGTTGGGGGTCCCCATACACAGGTAGATAAGTTGCCATATTGATCTAATCGGCAGCTTAAATCTG
CCTGTGTATGGCCTAGTTGCCTTCATACAAGTTAAATCTTTTTCTTTTATCATTTGACAAACTTAGTGTATAATTGCTGGTATAAATCTGGTGCTGTATA
GCTTTAAATTCCCTATTTATTACCAGGTAGGTGGCTTATGCCATATATAATTCTAAATACAAAATACAATTAACATTTCATTGTTTCATGCAGGAATAGA
TAGTATTTTATATCTATTATGGAGTAACTCATGCACTGTTATCTTCAGAAATCCATTTGCACTTGACATGCTGTACAGATGGTACTGAACCAAGTTTTGC
TTTTTGTGTATATTCAACTGTATGGGGACATATTTATTTCTAGTAAATATACTTCTGAGCTCTCGGAGCTTTATCACAGTATCATAGAGCAATATTTTGT
CTTTCACGGTGTAACTTGCTAATCACTGCCCCATCTTAATAAAGCTGCAAATGTGCACATACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF786148 [+]taattaatccccccccccccccgagaagtcaccaagagctgatgaacttctgcttcattatgatttgtgtctctttcatgggcaaaaggtattctgagta
acaccccgacatcctaaggggaccctatttttttcctgtaatcgttatgaagggatcagaagaagcctacctggtgagcgacaaagcaaccaagatgtac
tccttaactaaggacagtgaaaagaatcaccccagcaaacctcccctgcaggacgaagaaaacccacagagcaaatatcactgccacaataataacaaaa
aggcctacgatgccagacaaagagaacaaacgtttgccaaaaagaaactgtgtattgcatcacttatatgcttcgtatttatatctgcagagattgtagg
tgggtatatcgcaggaagcctcgctgttgtaaccgacgcagcccatctcttagtagatttgtctagtttctttattagtctctgttccctctggctgtca
tcaaagtcttcaacgacgcggctaacatttggatggcatcgagcggaaattctgggggcacttatgtccgttattacaatctggctggtgactggagtgc
tggtgtatttggcctgcgagcgattaattcgggctgactataccatcgatgggaccgtaatgctgataacgtccgcctgtggccttaaggggctaatttt
agtccttgggcttttgatttttgccccccaatccagggacccggaaccccagcccccgcccgggaagggaaaacaccgaaccattatgggccatcgggga
aattacaaaagccctccaaaacccaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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