XB-CLONE-4349790
Clone Name: IMAGE:7028375
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Gene:
gmppb
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080150 [+]
CCGGAATGGTACTGAGGTGGAAGGAGTCTGGTGGCAAGTGGAGCTGGTAGGTCAATATGAAGGCGCTAATTCTCGTTGGTGGATATGGCACTAGGCTGCG
TCCTTTGACACTGAGTGTTCCAAAGCCATTGGTGGACTTCTGTAACAAACCCATACTACTGCACCAAGTGGAGGCCCTGGTAAAGGCTGGAGTTAATCAT
GTGATCCTCGCTGTCAGCTACATGTCCGACATGTTAGAAAAGGAGATGAAGGAGCAGGAAAAGAGGCTTGGGATCCGAATCTCAATGTCGCATGAAAAGG
AGCCTTTGGGTACAGCTGGTCCTTTAGCCCTGGCCAGGGAACTACTAACCGAGAACTCCGACCCATTCTTTGTCCTAAACAGTGATGTCATTTGTGACTT
TCCTTTTGAGGAGATGGTGCGATTCCATAAACACCACGGGAAAGAAGGAACCATTGTGGTAACAAAGGTGGAGGAACCTTCAAAATATGGAGTTGTTGTA
TACGAGGCAGAGTCAGGGCAAATACAGCGCTTTGTGGAGAAGCCACAGGTGTTTGTGTCCAACAAGATCAACTCAGGACTGTATATCTTCTCCCCGGCTG
TGCTGGATCGTATTCAGCTACGTCCGACCTCCATAGAAAAGGAAATCTTCCCTGCTATGGCACAGGAGGGTCAGTTGTTTGCTATGGAGCTGCAAGGGTT
CTGGATGGACATTGGTCAGCCTAAAGATTTCCTGACTGGCATGTGCATGTACCTCCAGTCTGTCAGGCAGAAGCACCCAGAGTGGCTTCATGTGGGACCT
GGCTTTATTGGGAATGTTCTGGTGGATCCCACAGCTAAGATTGGTCAGAACTGCAGCATTGGCCCTAATGTGACTATTGGCCCTGGTGTGACTGTAGAGG
ACGGTGTGCGTATCAAGCGATGCACTATCATGAAAGGATCCAGGCTGCATTCCCATTCCTGGCTCGAGTCCTCCATTGTTGGTTGGAGCTCATCAGTAGG
ACAGTGGGTGCGCATGGAGAACGTGACCGTGCTGGGCGAGGATGTCATTGTTAATGATGAGCTTTACCTGAATGGAGCCAACGTCCTGCCGCACAAGTGC
ATCTCCGAGTCTGTACCCGAGCCACGGATAATCATGTAGAAGTGCAACGCTACTGCTCAGACAGCACTTCGCTCTATTTGGATTGGGGGCTGTGACCTTT
ATTCTGTGCTGTCCAGCAGTGAGGGTTTTGCGCTATAGTACAGAGGAGCACAGAAATAAAGAAGAGTATAATCTCAGGATATGGCATCCTCTACAATCAT
GGGTATACAGGCTGGCGCAGTGCTAAGAGTATGGCTAGTTTGCCAGTAGGCATGGGAGTTGAGGGACTGTCTGTCGGACTGTGGTGCCTTGGTTTCTTGA
TGAGCCGGGACAACTTGCTGGGAATCGGGCTGTTACCCCTCCAGATGTTCTGTGATATAAGGAGCAGCCTTCCCAGGGGCCTCAGATACTGAAAGGAACT
GCCAAACACTGTGCTCAGCCCAACTCTCCAACTTCCCATAACTGCTGCTTCTTCATGGGCCTAGAACTCAGTGGAAATTGCCAGTAATACCGGTGGCTGA
AGGCTACAATGAATATCAGGTACAGTGTGCCACTCCCATGTCTCACTGCAGCCTGTGGGTATTTAATGGAAGCAGGGTTTTAAAAGGATTTAAAATGCAG
ATTTCTCATACAGAGTGCCAGATGAATTTACTATGAGAACTCTACTGTGTCATTTGTGTTTTAAAAAAACAAACATCTTATAAAGAAGATTTTTAACTAC
CAAAAAAAAAAAAAAA
5' EST: CF786196 [+]taattaatcccccccccccccccggaatggtactgaggtggaaggagtctggtggcaagtggagctggtaggtcaatatgaaggcgctaattctcgttgg
tggatatggcactaggctgcgtcctttgacactgagtgttccaaagccattggtggacttctgtaacaaacccatactactgcaccaagtggaggccctg
gtaaaggctggagttaatcatgtgatcctcgctgtcagctacatgtccgacatgttagaaaaggagatgaaggagcaggaaaagaggcttgggatccgaa
tctcaatgtcgcatgaaaaggagcctttgggtacagctggtcctttagccctggccagggaactactaaccgagaactccgacccattctttgtcctaaa
cagtgatgtcatttgtgactttccttttgaggagatggtgcgattccataaacaccacgggaaagaaggaaccattgtggtaacaaaggtggaggaacct
tcaaaatatggagttgttgtatacgaggcagagtcagggcaaatacagcgcttttgtggagaaagccacaggtgttttgtgttccaaacaagattaaact
ccaggaaatggtatatcttttctcccccgggctgtggcctggaatcgcgaatttcagcttaacgttcccaaaactttcccttaaaaaaaaaagggaaaaa
tcttttcccccgggcataagggggccccaaggggaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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