XB-CLONE-4349674
Clone Name: IMAGE:7028457
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Gene:
dusp22
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084150 [+]
GAGTTGTTCCTGAGTGATACTAGGAAAGCTCACTATATCTTATCCCAAGAGCCCAGGCAGGCACATTCCCGGCAGCCGCAGCAGCAGCTAGAGGCAAGCA
CTGGGCAGCGCTAACTGGGCATTCCCATTAAGCAGGTGACGTTAGCGCAGTGTCAGTGGCAGCTTTGCTTCCTGTTGTGCCTGGAGGAACAGCCAAGTCG
CACGCAGCAATGGGCAATGGAATGAATAAGATTCTTCCCAGTTTGTTTATTGGAAACTTTAAAGATGCTCGAGATGTTGAACAGCTGCACAAAAACAACA
TTACTCATATACTCTCAATCCATGATAGTGCAAGGCCTATGCTGGAGGGCATGAAATATCTCTGCATTCCAGCCTCAGATTCACCAAGTCAGAACTTGAT
ACAGCATTTTAAGGACAGCATAGCATTTATACACGAATGCCGGCTAAAGGGGGAAGGCTGCCTTGTTCACTGTTTGGCTGGAGTCTCCAGAAGTGTAACA
TTAGTAGTAGCCTATGTTATGACAGTCACAGATTTTGGATGGGAAGATGCTTTGTCTGCCGTGAGAGGAGCAAGGACATGCGCCAACCCAAATATGGGCT
TCCAGAAGCAGCTTGAGGACTTTGGAAAACATGATGTCTACCAGTTCAGGACGTGGCTGAAAGAAACTTATGGGGAGAATCCTTTTAACGATAAAGATGA
TGCAAAACAGCTACTTGACAAACATAAACAGCAGGAAGCGGCAGAATCCCAAAGTGCCACAAGTTCTGGAAGGCAGTGGAGTAGCCACCTTACGTCTCTT
TCATCCATGTCTTATAGCAACTACACTACAGAGACTTAATAAGTCTGTATTGTCATTGCAGAATATGTCACTACTTAAAGACCATAAGCAGAGACCTTGT
TGGTGTTGGGCATTAGTTGTGATGAAGAGCGTAAATATGCCTTGATGAATCAGAATTCTGCTCTTAAGATTACAGTATTCTCTCCCTTTGTTGTTGAGAA
AATGGGTTAATTCAGCTACTTTTGTATTTTTTGGCATCTTGGCAATTTATATGGTATGATTACACAAATTAGCCCCTTAGAAATTTTTTAAAGATGAGCA
CTGTTGTTATTTTATTTGCTATACTTTAGCCTTTACCACATAGTTTTAACTTTTCTACCTCAGAATGCCATTGTTTCTATAGCTACATTAGTTCCCACCA
ACTGCGCCCTTAACCCATGACAAAGGGATCTGCTCCAATCACAGCATTGCTGGACTTCCTCAGGTAGTGTATATACACGGAACACAGTCGCATCAGGTGT
TTCTGTAATCTCTGAGACCAACATCAAATTACCAAGGTGTCATAACATGCTCAGGTAAATTACATTAGAATGTTTTAATTGCTTCTTTGAAAGCTGTGTG
GAGCATTTAGCATTTGCTATATGTAAAGTTGCTATTAATTACTTTATAACTGAATTACTATTAAGACATAAAGTTGGAGAGAGAGAGAAAGAGAGAGACT
ATGGCAAATGCCACTTTCTGACCATATTAAAATTTAGTAAAGGAAAAGCAAAGTTGTGTTTAACTATTGACAGTTTGTATACCATCATTTTCTTAATCCA
TTCAAATAGGTATTTATATAAATGTAATGTTAAAGAGCATGCTTGAATAATGGCATATTTATTTTGCATTACACTGAGTAATATGAGTGAAAAGTAACTG
TAACACTCTGGCCCTGGTGGTTATCTGCAAGGCCACTGAAAGTAACATCCTGGCATTTCCTGCTCCTGAATTCCCTTAGGCACCTGCCTCATTCCAGTGC
TGGTTCACTGTGTCCTCCTCCGGCTGTACCTAAAAAAGTGGAACCTGGCAGGTCGCACAAATGCATGTACAGTTTGTCATATGTGGTGTCAACATTTTTA
ATAAGTGACACATTTTACTCTTTTTGATGCAGCCCTAAATCTGGACTATAGACAGAACATTTACCAGTATATCCTATACCGCTACTACTACTAAAGAATT
TCCATGTATTATACAACTTTGTATGCTGTACATATGTATGCAGTGGTAAAATCTTTATGAGAATACAAAGAAAACATGTTATAAATGTTCCTAATTAAAA
GGAAATTACTGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF786254 [+]taattaatcccccccccccccgagttgttcctgagtgatactaggaaagctcactatatcttatcccaagagcccaggcaggcacattcccggcagccgc
agcagcagctagaggcaagcactgggcagcgctaactgggcattcccattaagcaggtgacgttagcgcagtgtcagtggcagctttgcttcctgttgtg
cctggaggaacagccaagtcgcacgcagcaatgggcaatggaatgaataagattcttcccagtttgtttattggaaactttaaagatgctcgagatgttg
aacagctgcacaaaaacaacattactcatatactctcaatccatgatagtgcaaggcctatgctggagggcatgaaatatctctgcattccagcctcaga
ttcaccaagtcagaacttgatacagcattttaaggacagcatagcatttatacacgaatgccggctaaagggggaaggctgccttgttcactgtttggct
ggagtctccagaagtgtaacattagtagtagcctatgttatgacagtcacagattttggatgggaagatgctttgtctgccgtgagaggagcaaggacat
gcgccaacccanatatgggcttccagaagcagcttgaggactttggaaaacatgatgtctaccagttcaagacgtggctgaaagaaacttatggggagaa
tccttttaacgataaagatgatgccaaacagctacttgacaaacataaacagcaggaaagcggcagaatccccaaagtggccacaagtttcggggaaggc
cagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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