XB-CLONE-4349558
Clone Name: IMAGE:7029187
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Gene:
MGC89871
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077031
[+]
GAGTGGTCAGGAGAGGAGCGGAGCGACAATGAACAACAAATTCGATGCTCTAAAAGATGACGACAGTGGGGATCATGATCAGAACGAGGAAAATGGCACA
CAGAAAGACAGTGAGAAGGAAAAAAATGAGAAGGAAAAAAACCAAGGCTCTTCTAGGAAAAAGAGTGTTGTGCCAGGTCCAGCCGAGCACCCCTTACAGT
ACAACTACACATTTTGGTACTCACGCAGAACTCCAGGAAGGCCAACCAGCTCCCAGAGCTATGAGCAGAACATTAAACAGATCGGTACCTTTGCTTCTGT
GGAACAGTTTTGGAGGTTCTATAGTCACATGGTACGTCCTGGGGACCTGACTGGGCACAGTGACTTTCACCTCTTCAAGGAAGGGATTAAACCAATGTGG
GAGGATGATGCAAACAAAAATGGAGGGAAGTGGATAATTCGCCTTCGCAAAGGCCTAGCATCACGATGCTGGGAAAATCTCATACTGGCCATGCTGGGAG
AGCAGTTTATGGTTGGAGAGGAGATCTGTGGGGCTGTTGTCTCTGTGCGTTTTCAGGAAGACATCATATCTATTTGGAACAAAACTGCAAGTGATCAGGC
AACCACTGCTCGTATTCGAGACACATTGCGGCGAGTCCTGAATTTGCCTCCCAACACTGTTATGGAATACAAAACGCACACAGACAGCATCAAGGACAAA
ACAAGCTTCAGAAATACCAAAATTGCTTTGTGAACACAAGGATCTACTCCTGCTTTGCTGACCTGCTTTCAGTTTGAGTATTCTCAGTTTTAAGCACCAC
CATGTAGCTTTATGGCCGGTGGCCCAACGGTTCTTGGGAAAAATTCTATACAGCTTCAAATCTAGCTGGGATTGGGTCGAACTGTCTTTTTGTTGTTTTT
GTTTTTTTTTAAGCCTGTTATCTACATCAATCCTCTTTCCAAGACCTATCCACTACAGCTGCATCCCTCACGGGAGGAAAAAGCATTGTACATTATTTTT
GGAATCTGTTTTATTTCTTGGTTTTATTTCATTTTTAAATGATGGAAGAAGATTGGCATTGAACTTTTCTTTTGTATGTAGAGTCCTCTTCAGCACTGAT
GGAGTTAAGTGCTCCCTTCTGTTGAGAAGCAACCCTTTCTTCTCAGCATTTGCCTTGTCCCTTTTCTTCAGCACTTTGTTACTTTCCTTTCCATTTGTTA
TGCAAAGGATTATGTGTATAGTAGTAATCATATTACCTAACCCATTACTGAGACATATCAGTATTATACTTTGTGTATATAGAATAAAATGTCTGTTTGC
TGATGTCTGCAGCTGTCTCTCCTGCTACAGCTTTGACATTAAATCATAAGATCTCTTTGGTTTTGTTTTTATAATGAAAAAAAAAAAAAAAA
5' EST: CF786312
[+]taattaatcccccccccccccgagtggtcaggagaggagcggagcgacaatgaacaacaaattcgatgctctaaaagatgacgacagtggggatcatgat
cagaacgaggaaaatggcacacagaaagacagtgagaaggaaaaaaatgagaaggaaaaaaaccaaggctcttctaggaaaaagagtgttgtgccaggtc
cagccgagcaccccttacagtacaactacacattttggtactcacgcagaactccaggaaggccaaccagctcccagagctatgagcagaacattaaaca
gatcggtacctttgcttctgtggaacagttttggaggttctatagtcacatggtacgtcctggggacctgactgggcacagtgactttcacctcttcaag
gaagggattaaaccaatgtgggaggatgatgcaaacaaaaatggagggaagtggataattcgccttcgcaaaggcctagcatcacgatgctgggaaaatc
tcatactggccatgctgggagagcagtttatggttggagaggagatctgtggggctgttgtctctgtgcgttttcaggaagacatcatatctatttggaa
caaaactgcaagtgatcaggcaaccactgctcgtattcgagacacattgcggcgagtcctgaatttgcctcccaacactgttatggaatacaaaacgcac
acagacagcatcaaggacaaaacaagcttcagaaataccaaaattgctttgtgaacacaaggatctactcctgctttgctgacctgctttcagtttgagt
attctcagttttat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: