XB-CLONE-4349522
Clone Name: IMAGE:7029074
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Gene:
smad3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080156 [+]
GGAAGCACAGAGAGGGACAGGGAGCGACTGAGGCCACAGTACGGGCAGAGAGTGCACAGTAAGAAGTATATATATATCTAGAGAGAGAGAGAGAGGGCAA
AAGAGAGAGTCGTGGTAATAGCTGCTGAGTAAAGTGTGTGTGCGCCAGTGGGGAAGAGTCCCAAGGAGCCTGAGTGACAATAGGTGTGTGAGCTGCAGCC
TGACAGGGATTGTCCCCTTGTCTCCCCCGCGGCCCCCCAGCTCCTGTCCTTTCTCTCCTATTATATCTCTGTTCTGCTTGGGGGGCTGTCATGTCCTCGA
TTCTTCCCTTCACTCCCCCCATCGTGAAGAGGCTGCTGGGATGGAAGAAGGGAGAGCAGAATGGGCAAGAGGAGAAATGGTGTGAGAAGGCAGTGAAGAG
TCTGGTGAAGAAGCTGAAGAAGAGTGGGCAGCTGGACGAGCTGGAGAAGGCACTGACCACCCAGAGCATCAGCACCAAGTGCATCACCATCCCCAGGTCT
CTGGATGGAAGGCTCCAGGTGTCCCATCGCAAGGGTTTGCCCCATGTCATATACTGCCGCCTTTGGAGGTGGCCAGATCTACACAGTCACCACGAACTTC
GAGCCATGGAGGTGTGTGAATATGCCTTCAACATGAAGAAAGACGAAGTGTGTGTGAATCCTTATCACTACCAGAGAGTGGAGACTCCAGTTCTACCTCC
AGTGCTGGTTCCACGAAACACCGAAATTCCGGCAGAATTCCCTTCACTCGATGATTACAGCCATTCCATTCCAGAAAATACCAATTTCCCGGCAGGCATT
GAGCCACAGATAAACTACATTCCAGAAACTCCTCCTCCTGGTTACTTGAGTGAAGATGGGGAAACAAGCGACCAAATGAACCACAGCATAGATACAGGGT
CTCCAAATTTGTCTCCAAACTCTATGTCCCCTGCTCATAGCAACATGGACCTGCAGCCTGTTACATACTGCGAGCCGGCCTTTTGGTGCTCCATCTCCTA
CTATGAGCTTAACCAACGTGTGGGGGAGACCTTCCACGCTTCCCAACCCTCCATGACAGTGGATGGATTCACCGATCCTTCCAACTCCGAACGTTTCTGC
CTGGGACTGTTGTCCAATGTAAATCGGAATGCGGCTGTGGAAATGACACGGAGACACATTGGGAGAGGCGTGCGGCTGTATTACATCGGAGGGGAAGTGT
TTGCTGAGTGCCTCAGTGACAATGCCATATTTGTACAGTCCCCAAATTGTAACCAGCGCTACGGTTGGCATCCTGCCACGGTCTGCAAGATTCCACCAGG
CTGTAACCTGAAGATATTTAATAACCAGGAGTTTGCTGCTCTTTTGGCTCAGTCAGTAAATCAAGGCTTTGAGGCTGTGTATCAGCTCACTAGGATGTGC
ACCATACGCATGAGTTTTGTCAAGGGCTGGGGAGCCGAATACAGGCGACAGACTGTGACTAGCACCCCCTGCTGGATCGAGCTGCACTTGAACGGGCCCT
TGCAATGGCTGGATAAAGTTCTCACTCAGATGGGGTCTCCAAGTATCCGCTGCTCCAGTGTTTCTTAAAGAGAAGCAGCTTGGTGACGGGATATAGCATT
CTGTGGGCCAAGCGATGAAAGAGACTAAGCTTTAGGACTAAACAAGTCTATTGCGTCTTAGTCCATTCAGACCATGCTTAGACCAGAGACAGCCAACTGA
AGCCAAGTAACTGAAGTACGCAAACTTATCAGTGGAACAACATCTCATTGGCCAAGTTCAAGGACTTGTAATCTAGAACTTACCAGCACTCCAATATCCA
GTATGGTGGTGCAATAAAAGCTGGCTCTTACTGCACACCATTATCCAGGATAACCAAACTGCTGCTGTCCTTGTACACCTCACTTGCAGACGCATGACTT
AGAGCCAGTGGACCTGGCCTGTATTAAAAAAAAAAAAAAAA
5' EST: CF786330 [+]taattaatcccccccccccccggaagcacagagagggacagggagcgactgaggccacagtacgggcagagagtgcacagtaagaagtatatatatatct
agagagagagagagagggcaaaagagagagtcgtggtaatagctgctgagtaaagtgtgtgtgcgccagtggggaagagtcccaaggagcctgagtgaca
ataggtgtgtgagctgcagcctgacagggattgtccccttgtctcccccgcggccccccagctcctgtcctttctctcctattatatctctgttctgctt
ggggggctgtcatgtcctcgattcttcccttcactccccccatcgtgaagaggctgctgggatggaagaagggagagcagaatgggcaagaggagaaatg
gtgtgagaaggcagtgaagagtctggtgaagaagctgaagaagagtgggcagctggacgagctggagaaggcactgaccacccagagcatcagcaccaag
tgcatcaccatccccaggtctctggatggaaggctccaggtgtcccatcgcaagggtttgccccatgtcatatactgccgcctttggaggtggccagatc
tacacagtcaccacgaacttcgagccatggaggtgtgtgaatatgccttcaacatgaagaaagacgaagtgtgtgtgaatccttatcactaccagagagt
ggagactccagttctacctccagtgctggttccacgaaacaccgaaattccggcagaattcccttcactcgatgattacagccattccattccagaanat
acaatttcccggcagcatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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