XB-CLONE-4349486
Clone Name: IMAGE:7029006
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Gene:
erp44
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084525 [+]
GTCCGGTTGCGCTAGTAAGGGCTCAGTAACATCACCGGCATATCTCTATGAATATGAGACGCGGTATCTTCGTCCCTTACTTTTGCCCGGCATACCTTCT
CATGCCATTGGTAATGTGGGTTTACACTCCAGTAAAAAGTGAAATACTTACCCTACAGAGTGGCAATATAGATGATATTTTAAGAAATGCTGATGTTGCT
TTAGTAAATTTCTATGCTGACTGGTGCCGCTTCAGTCAGATGCTGCACCCTATATTTGAAGAAGCATCTAATATAATACAAGAAGAATATCCTGATAAAA
ATAAAGTTGTTTTTGCTAGAGTGGACTGTGATCAACATTCTGATATAGCACAGAGATACAGGATCAGTAAGTATCCTACACTAAAGCTTTTCAGAAATGG
AATGATGATGAAAAGAGAATACAGGGGGCAACGCTCCGTTACTGCAATTGCTGACTATATTCGACAGCAAAAGAGTAATCCTATACGTGAAATTGGTGAC
CTGGAAGAATTAAAAACTGTTGATCGGAGTAAAAGAAATATAATTGGCTTTTTTGAAAGCAAGGAAATAGACAACTTCAGGACCTTTGAAAAAGTAGCAA
ATATTTTGCATGATGATTGTGTCTTCCTCGCTGCATTTGGTGATGTAGCAAAGCCAGAGAGGTTTAGTGGTGACAATGTAATCTATAGACCAATTGGGGA
GAGTGCTCCAGACATGGTTTATTTGGGATCATTAACCAACTTTGACCTGACATTTGCTTGGACACAAGATAAATGTGTCCCTCTTGTTAGAGAAATTACA
TTTGAAAATGGAGAGGAGCTGACAGAAGAAGGTCTTCCGTTTCTAATACTTTTTCATTTGAAAGATGATACTGAAAGCCTAGAAAAATTCCAACAAGAGG
TTGCTCGACAACTAATAAGCGAAAAAGGAACAATTAATTTCCTACATGCAGACTGTGAAAAATTCAGGCACCCTCTTCTGCACATACAGAAGACACCTGC
TGATTGTCCTGTAATAGCCATTGATAGCTTCAGACATATGTATGTCTTTTCAGACTTTAAAGATTTGTCAATTTCTGGTAAACTTAAACAATTTGTACTA
GACCTGCACTCTGGAAAACTACACAGGGAATTTCATCATGGACCAGACCCTACAGATGTTGCACCTGACCAGCCAACTGAAGATATAATAAGTAATCCTC
CAGAGAGCTCCTTCCAGAAACTGGCTCCAAGTGAACATAGGTACACTATACTAAGAAGAGATCGTGATGAACTTTAAGTAAATTGAACTGCCTTGGTAGC
CTTAAAACAAAAAAAGATCAATTTCTGTATGGTGAAAAAAATGTTAACCTATATTTTCATATTTGTATTTTTTATTTGGGAAAAAAACAAAACAAAGTAA
TAAATTGTTTATTTTATTTGCTCCCCAAGATTGTAGATATGTTTGTGTTTTGTGGATTTCCTCTGTAGAAAATTTTAAACTCTTACAAAACCGTTCTTTC
AAAAAAGGCTTCCCTGTTTTTTAAATCATACAAAATTAAAACAAGGAGACAATACTGTTAACTTGCTCATCCAAATTAAAATTTGAGATTTTTGATCTTT
AAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF786348 [+]taattaatcccccccccccccgtccggttgcgctagtaagggctcagtaacatcaccggcatatctctatgaatatgagacgcggtatcttcgtccctta
cttttgcccggcataccttctcatgccattggtaatgtgggtttacactccagtaaaaagtgaaatacttaccctacagagtggcaatatagatgatatt
ttaagaaatgctgatgttgctttagtaaatttctatgctgactggtgccgcttcagtcagatgctgcaccctatatttgaagaagcatctaatataatac
aagaagaatatcctgataaaaataaagttgtttttgctagagtggactgtgatcaacattctgatatagcacagagatacaggatcagtaagtatcctac
actaaagcttttcagaaatggaatgatgatgaaaagagaatacagggggcaacgctccgttactgcaattgctgactatattcgacagcaaaagagtaat
cctatacgtgaaattggtgacctggaagaattaaaaactgttgatcggagtaaaagaaatataattggcttttttgaaagcaaggaaatagacaacttca
ggacctttgaaaaagtagcaaatattttgcatgatgattgtgtcttcctcgctgcatttggtgatgtagcnaagccagaagaggtttagtggtgacaatg
taatctatagaccaaattggggagagtgctcccagacatggttttatttggggatcatttaaccaacttttgacctggaattttgcttgggaacacaaga
ataaatgtgttccctctttggtaga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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