XB-CLONE-4349394
Clone Name: IMAGE:7028784
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Gene:
depdc1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081375 [+]
GGGGGTCACGTGGGCTGAGGGTTAAAGGGCTGCAGGCGGCTCACGTCTCTTCGTTGGTCCGGGTATGGACAGCCGTATCATTACCCCGGGGCCGTACCGG
GCCACCAAGCTGTGGAATGAAGTAACAAAACATTTTCGGGCGGGGATGCCACTGAGAAAACACAGACAACACTTCAAGGCCTATGGAAATTGCTTTACAG
CTTCTGAAGCAATAGATTGGCTCCATCACTTGCTAAAAACCAACAGCAATTTTGGTTCTGAGGTGACACGGCAGCAGACCATCCAGTTACTGAGAAAGTT
CTTAAAAAACCATGTAATTGAGGATCTAAAGGGAAGATGGGGAACGGAGGATCTAGAAGACAACAACAGTCTTTACAGATTTCCTTCCACTTCCCCAGTT
AAGACCATTCCAAGCCGTCCTCCACTGTGGAAAAGAAGCAGTTTAGAAAATGTATTTAAAGAAAGAGAACCCATATTCAAGATGCCGCAGTTCTCTAAAA
AAACACCTAAAAGACCTGGTTCTGTGGATTCTAAGGAAGAGCAAGAAAATGAGGATGTGACGGAAAACCAAGGGAATGATGATTTTCCTAAGCAGCTGAG
TAGCAAGGATATTGATGACATTTGGGGAAATATAACCTTGATACACCTTCAGAAAATACTCTGTTTGCCATCGTTGGAAGAAGTTTTAAATCCTGCTCAG
ATAAAGCCTAATTATATAAGGTATAATATGACAAATACCAGTAAGCATGGAGTTGTTGTTCTTCAAGACAAAACAGATGACCTTCCTCACTGGGTGCTGT
CTGCTATGAAATGCCTTGCCAATTGGCCAAAGAATAATGACATGAGTCAATCAACATATATTGGTTTCGAACGTGATGTTTTTAGGACAGTTGCTGACTA
TTTCCTAAATCTTCCAGAGCCTCTGCTCACATTTGAATTTTATGAGCTTTTTGTTAATATTTTGGTTGTTTGTGGCTACATAACGGTTCCCAAAAGTCAT
AATGGAAAACATAGATTCCATAATAAAACACTCGATCCAGCACCAGCTAAAAAACAACACTTAAATGATGAAACCTTTTTCAAGTCAACAGAATGTCTTC
TTTTAAGTTTGTTACGGAAGGTCCCCAATGAAGAAGAGGAGGATGATGTTGAGCATAGTTTTGGGGAGATTGCCAGAGACAACAAAGACCAAATTCCTCA
AGATGTATTTCCTAAAAAGACTCCTTTGTACAGTTCCTTCAGTAGAAGATCCAAAAGGGGAATTATTGGAGGGAGTTGCCAAAATCTTTCTACTTCCAGG
AAAGAACCTGATGTGATCCACAAAGTAAGGTTACGTAGCTGCTCCCTGGAAGGTATAGCTGATAGTGTGTATACAGACAACAATGACTGTGAGAACAAAG
TTCTGCAAGCTTCCTCGCAAAGACCAGAATGCAGAGCTGTGAAAGAGAGTCCAATAACATCTGTAAAGAAAGCTGAATCTGTGACTGGCTTTTATGCTCC
TCATGATAAACTGCACTACAGGACTAGTGATGGGGGCTACTCCCAAAACAGTGAAAAGCTTGCTAGATCAGTGAGCATGGGGGATTGTCTTGGGTCTCGT
ACAAGTAAAAATGCACCGGTTGCTGAGATCACTATTAAACCTTTAAAATCTGCACAAACTAGAATGCAAAAGCGTTTGTCTTCTATGGACAGTGAACCTG
CAGATCTGAATTTCACCGTAACAAAGAGATTATGTCAGAGTACCATGGAACTCTCAAATTCTTCTTTACCTTCAACTTCTTCTTTACTGCCACATACTAC
TACCCCCAACAGCTCTGGCTCGGCAAGCTTGTTGCAGCCACACTTGGAGAGAGTTGCAATTGAGGCACTGCAACTTTGCTGTCTGCTGCTTCCGCCTGCA
AACAGACGAAAGCTCCAACTGTTAATGCGCATGATCTCTCGCATGAGTCAGAATGTGGATATGCCGCGGCTTCATGATGCCATGGGGACAAGATCCTTGA
TGATTCAAACCTTTTCCCGATGTGTCCTATGCTGTGCTGAAGAAGTGGATCTGGATGAATTGCTTGCATCAAGGCTGGTTTCCTTCCTCATGGATCATCA
CCAAGATATCCTACAAGTGCCTTGTTATTTGCAAACTGCTGTCCAAGATCATATACAGTATCTTCAAAGACCCCGTGTAAAGCAAGGCACTGTGCCATCC
TATTACTTTTGTAAGCAGATAAGCACCCAAGAGTTTGAAGAGCAGAGATTGGCCACCTCTCAAGCTGCTATGATGGAACTTTTAGAAAATATTGTAAGAG
ACAGAAATTTAGCAGTTAAAGACAAAAAGAAGAAGCTAAAGCAGAAACAATACCCAGATATCTACCACAACCGGTTTCCAACCACTGAAAGTGAAGCCAA
ATTGTTTGGAGATAAACCTACAATCAAACAACCAATGTTGATGCTGAAGAAGCCAAAGTTTAAAAGCCTGCGATACTGACTAGAAAATACACACATTTAA
AAAAAAAAAAAATTGTATAAATCCAGTTATTTTATGTACTATGAATTGTATCTTTGCAAGACATCAGTGCTATCTTCAGCTATGTTATATAATGCTTTGT
GTGAATCTCTGACGGCTTCTTTGTACTGGTAATCAGCCTCTTCTGCAAATCATGGATACATAGGAGTTTCAGGCTGTAAATTCCTGCCTGTAGTAACTCT
TTAGATGCCACTCTAGTGCACTGGAGAAGGTGATATGCAGTGTCTTATAGGACAATCATGGGTAACTGTTAGGTTGTCTACAATAATAATGTATAACCAC
TTCCCAAGCACCCAAAATATGTATGATTCTTATTGCAACCTGTTAGCATTTTGGCTATTTTCTAAGGCTCGGTTAATGTCCCTTCCAAACTAAGACAAGC
TCTCCTTGGACCCAGCACACACTCCTTAAACATCTTAAACTCCATTAACATTAGCCTCATGTGTAGAATAATTATACAGGGATGCTTGATGATGGCAGGG
GGGCGGCAGCTACCCTCAAAGAGAAAATTTGTCTTTATGGGTCATTTTGAGCCCAGACACACCCACACACCTTATTCCTAACCCATTTTAGTTTGTGGTT
TTTTTTTGTTTTTTTTTAATATGGCATAAATAACAACAAAGCTCATGTGCCGTGCTGAGCCTAAATGCGATGCAGAGGGTATATTATACTATACACTGCT
TGTGCTATATGTACCGGTAATGCCTGATACAGACTAAGCCTTCCAATTGAAAACTTTGAGTTAACAGTCAAGATTCAAACTTCTGTGTGCCTTAAGAGAG
GTACTAGTCTTAAGCTTGGTATTAGCAAGTTCTGTGTGGAAGTATACTATATAACTTATAAAGACAAAAAAAAAAAAAAAA
5' EST: CF786394 [+]taattaatccccccccccccgggggtcacgtgggctgagggttaaagggctgcaggcggctcacgtctcttcgttggtccgggtatggacagccgtatca
ttaccccggggccgtaccgggccaccaagctgtggaatgaagtaacaaaacattttcgggcggggatgccactgagaaaacacagacaacacttcaaggc
ctatggaaattgctttacagcttctgaagcaatagattggctccatcacttgctaaaaaccaacagcaattttggttctgaggtgacacggcagcagacc
atccagttactgagaaagttcttaaaaaaccatgtaattgaggatctaaagggaagatggggaacggaggatctagaagacaacaacagtctttacagat
ttccttccacttccccagttaagaccattccaagccgtcctccactgtggaaaagaagcagtttagaaaatgtatttaaagaaagagaacccatattcaa
gatgccgcagttctctaaaaaaacacctaaaagacctggttctgtggattctaaggaagagcaagaaaatgaggatgtgacggaaaaccaagggaatgat
gattttcctaagcagctgagtagcaaggatattgatgacatttggggaaatataaccttgatacaccttcagaaaatactctgtttgcccatcgttggaa
gaagttttaatccttgctcagataaagcctaattatataaggtataatatgacnaataccagtaagcatgggagttgtttgttcttcaagaacaaaacag
atgacctttcctcactg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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