XB-CLONE-4349366
Clone Name: IMAGE:7028720
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Gene:
calm2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082735 [+]
CGAGTCCGAGCCGAGCCTGCGAGGGTTTAGGTTGCGTCGGAGACATTGTGCGCGTCTGGTCAGAGCTTCTCGATTATACTAACGCAACTATTCCGAAATG
GCTGACCAACTGACAGAAGAGCAGATTGCAGAGTTCAAAGAAGCATTCTCATTATTCGACAAGGATGGGGATGGTACCATCACCACAAAGGAACTTGGCA
CTGTTATGAGGTCGCTTGGACAAAATCCAACAGAAGCAGAATTGCAGGATATGATCAATGAAGTAGATGCTGATGGCAATGGAACAATTGACTTTCCTGA
ATTTCTGACTATGATGGCTAGAAAAATGAAGGACACAGACAGTGAAGAAGAAATCAGAGAAGCATTCCGCGTGTTTGACAAGGATGGGAATGGTTATATC
AGTGCTGCTGAATTACGTCACGTGATGACAAACCTTGGGGAGAAATTAACAGATGAAGAAGTTGATGAAATGATAAGGGAAGCAGATATTGATGGAGATG
GCCAAGTAAACTATGAAGAGTTTGTACAAATGATGACAGCAAAGTGAAGAGTGTACAGAATGTGTTTAAATCTCTTGTACAAAGATGTTTATCTGCCTTT
CTTTGTTTGTAACTTATCTGTAAAGTGTTCCCTTACTGTCAAAAAAATGCATGTATAGTAATTTAGAATTCCATCCATGTTTCCCATCTTGTCATAATTT
TTTTTTTTTTTTTTTGTTTATTTTGTTTTTTAAATCCAAGTATCTAGTCTGCTTGTTGGAATGTGGAGTAAACAACTCTAAAACTACAGATCATTAACAA
TATTGAACTTATGTTGCACTAATGCAAAACTGGTAAATCCAGGTACTTGTCTCGTACACACTTTTTTTTTCCCCTCCCCTTCCCAGCTCCCCCCATACTT
TTCCTGACCTGTGTACGGAACATTCACATTGCTTTTTTTCTTTTTTTTTTTTCTTGTTCAACTGCATTCACTGGTGAGAGCTCTGCAGAACAACATTGTT
CAGCACAGAGTTCCTCTGCTGGTATAAAAAAAAAAGTTTACTTTTTGTTTTCATTTCCTTTTGTTTAGCCACTTCCTTCCTTTAAACCTGCTTATGGCAC
AATTTGCCTCAAATCCATTCCAAGTTGTATATTTGTTTTCCAATAAAAAAGACAATTTACTATGAAAAAAAAAAAAAAAA
5' EST: CF786408 [+]taattaatccccccccccccccgagtccgagccgagcctgcgagggtttaggttgcgtcggagacattgtgcgcgtctggtcagagcttctcgattatac
taacgcaactattccgaaatggctgaccaactgacagaagagcagattgcagagttcaaagaagcattctcattattcgacaaggatggggatggtacca
tcaccacaaaggaacttggcactgttatgaggtcgcttggacaaaatccaacagaagcagaattgcaggatatgatcaatgaagtagatgctgatggcaa
tggaacaattgactttcctgaatttctgactatgatggctagaaaaatgaaggacacagacagtgaagaagaaatcagagaagcattccgcgtgtttgac
aaggatgggaatggttatatcagtgctgctgaattacgtcacgtgatgacaaaccttggggagaaattaacagatgaagaagttgatgaaatgataaggg
aagcagatattgatggagatggccaagtaaactatgaagagtttgtacaaatgatgacagcaaagtgaagagtgtacagaatgtgtttaaatctcttgta
caaagatgtttatctgcctttctttgtttgtaacttatctgtaaagtgttcccttactgtcaaaaaaatgcatgtatagtaattttagaattccctccat
gtttcccatcttgtcctaaattn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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