XB-CLONE-4349302
Clone Name: IMAGE:7030070
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Gene:
elavl3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080177 [+]
GTTCAGATTGAGCAAGGGAACGAGGTGTCACCACACTGGGATAACGGCACCAGGAGAACCATTTCTACCCCTCCCTTTTCCCTCTCTCTCCAAAATCTTG
CAACCCCCTCCTCCTCTTTCCAGACAAAGACCCCCCCAACTTACTACTGACACTATCCCTCAATTCCTCTTTAGCACCGGTTTTTCCGTCACTCATTTCT
ATATATATAATTCCTATTTTGGGGATTTCTTCCCACCTCTCCCAATTTTGTACCATTTTTGGAATTTATTGCCCCTTCTCACCAGCTGAGGGAGTATTTT
GATACCATTATTTTTCACAATTCGGTTTTCTGATTCGATCCCGTGACGGCCGCCGGGAGGGAGCAGGGGAGCGGCGGGGTGGGCAGGCGCACCTCGGACG
CCAGGACGCTGAGGAAAGGAACAAGAAGGCTAGAGAGGCGCAACCGAAAATCTACAGGATCCAACCTACGTTCCCTAAGCATGGTGACTCAGATAATCAG
CACCATGGAGACCCAGGCCAGTAATGGGCCAGGAAGTGTAGGTATCCTCAATGGCACCAACGGGGCAGCCGATGACAGCAAAACCAACCTGATCGTCAAC
TACCTCCCTCAGAACATGACCCAGGAGGAATTCAAGAGCCTGTTTGGAAGCATAGGAGAGATCGAGTCCTGCAAGCTGGTCAGGGACAAAATCACAGGTC
AGAGCTTGGGGTATGGATTTGTAAACTATGTTGACCCAAATGATGCAGATAAAGCAATAAACACCCTAAATGGACTGAAACTACAGACTAAGACCATCAA
GGTCTCCTATGCTCGTCCCAGCTCTGCCTCCATCCGAGATGCCAATTTATATGTCAGTAGCCTTCCGAAAACTATGAACCAGAAAGAAATGGAGCAGCTC
TTTTCTCAGTATGGCAGAATCATAACCTCCCGTATCCTGGTGGATCAAGTGACAGGTAGTGTTTCCCGAGGTGTGGGCTTTATTCGGTTTGATAAGAGAA
TAGAGGCAGAGGAGGCTATTAAAGGCCTTAATGGACAGAAACCACTTGGTGCCAGTGAACCAATCACAGTCAAATTTGCCAACAATCCCAGTCAGAAAAC
AGGACAGGCGCTACTCACTCACTTGTACCAGACTACAGCTCGGAGGTACACAGGACCCCTGCACCATCAAACTCAGAGATTCAGGTTCTCACCTATCACC
ATAGACAGTGTTACCAACCTTGCCGGCGTCAGCCTTACTGGTCCCACCACTGCTGGTTGGTGCATCTTTGTCTACAACTTGTCCCCTGAGGCAGATGAGA
GTGTCCTGTGGCAGCTGTTTGGACCATTTGGAGCCGTGACAAACGTCAAAGTGATCCGGGACTTCACTACTAACAAGTGCAAAGGCTTTGGATTCGTCAC
AATGACGAACTACGATGAAGCTGCCATGGCTATCGCTAGTCTCAACGGATACCGCCTGGGAGACCGGGTCCTGCAAGTGTCATTCAAAACCAGTAAACAG
CACAAGGCCTGAAGGGAAATATCGTAAGGAAAGGGAGTGAGAGGCAGGGCAGGAGTGGGGAGAAAAAGAGAGAGAGAAAAAGAGAGAGAGGGGGAAGGAT
TGAGATAGAGGGAAATAGGTGTTGTGAGGAAGGGGAGGATAGAGGAGGAATAGGAATCAAAAAACAAATATGGAGATAGAGTCACCTCTAAGCCTGTGTC
CCAAAGCAAAGAGTCTGGCACAGGACATTGAGCGTGACGTCTTGGTTTAGAAACCAGCCTCTTTGTGGTTCCAAAACAGTTCCAATTCCTGTGGCTGGAC
ATGGGAAATCCTAAAGTAGGGCTAAGCAGTGAGGATATTTATCCTGACATGGCGGTCTAATAACGTTATTCTACTCTCTCTCTGGCAGTGAACGGGTTAA
TCTTCTGCCCTTCCAAACATCTAAAAAAAACATTAACCCATCTGCTGCCGTAACCATTTTCCAAGCTCCCTGCAAACCCCGCGCTCTTCACCTCCCTAAC
CTCATGCTTCTACGGAAAGGTGATTATAAACGAAGAAATGAGACGGGCGCAGACATTTAAAAAATATATATATATATTTTTATATAAAAAAGCATAAAAA
AAAAATCCCGCAAGCAGACAGAACCTTCATACCTGTCCAAAAAATAAGCCCACTGGACAGAATTGTGCTTTTAATTCCCTAATGTGGGTTCTGAAATAAA
AAAAAAAGAAAAGAAATCCTATTTTTATACAGAAAAAAAAAAAAAAAA
5' EST: CF786440 [+]taattaatccccccccccccgttcagattgagcaagggaacgaggtgtcaccacactgggataacggcaccaggagaaccatttctacccctcccttttc
cctctctctccaaaatcttgcaaccccctcctcctctttccagacaaagacccccccaacttactactgacactatccctcaattcctctttagcaccgg
tttttccgtcactcatttctatatatataattcctattttggggatttcttcccacctctcccaattttgtaccatttttggaatttattgccccttctc
accagctgagggagtattttgataccattatttttcacaattcggttttctgattcgatcccgtgacggccgccgggagggagcaggggagcggcggggt
gggcaggcgcacctcggacgccaggacgctgaggaaaggaacaagaaggctagagaggcgcaaccgaaaatctacaggatccaacctacgttccctaagc
atggtgactcagataatcagcaccatggagacccaggccagtaatgggccaggaagtgtaggtatcctcaatggcaccaacggggcagccgatgacagca
aaaccaacctgatcgtcaactacctccctcagaacatgacccaggaggaattcaagagcctggtttggaagcataggagagatcgagtcctgcaagctgg
tcagggacaaaatcacaggtcagagcttgggggtagggatttgtaaactatgttgaacccaaatgaatgcgataaagcaataaaccccctatatgggact
gaaaactca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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