XB-CLONE-4349274
Clone Name: IMAGE:7030014
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Gene:
tuba5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080160 [+]
CCGAGTTGCTGTGTTGGCGGAGACCCGTAGCGCGTCTATAGTCAACGGAGCCAAAGAGGGGCGAGTGTAGGAAGACTGAAGGTTATCTGGTCCTTTATTT
AGTTCTACCGGTGCGCACACATCTAATCAGAAAATGCGTGAGTGTATATCTGTCCATGTTGGCCAGGCTGGAGTTCAGATCGGTAATGCATGCTGGGAAC
TTTACTGCCTAGAACATGGGATCCAACCAGATGGTCAGATGCCTAGTGACAAAACTATTGGGGGTGGAGATGATTCCTTTAACACTTTCTTTAGTGAAAC
TGGAGCAGGAAAACATGTTCCTAGAGCAGTGTTTGTAGACCTGGAATCTGCAGTCATAGATGAGGTTCGTAATGGGACATATAGGCAGCTTTTTCACCCT
GAGCAGCTTATAACTGGTAAAGAGGATGCTGCCAATAATTATGCACGTGGGCACTACACTGTAGGAAAAGAGATCATTGACTTGGTGCTGGAAAGAGTAC
GGAAACTGGCTGACCAATGCACAGGTCTCCAGGGTTTTCTGATTTTCCACAGCTTTGGAGGAGGAACAGGATCTGGATTTACATCCTTGCTTATGGAGAG
ACTATCTGTAGATTATGGCAAGAAGTCCAAGTTGGAATTTGCCATCTATCCAGCCCCACAAGTGTCTACAGCTGTGGTTGAACCCTATAATTCCATTCTT
ACCACACACACTACACTGGAGCACTCTGACTGTGCATTTATGGTGGATAATGAGGCCATTTATGACATATGCAGGCGGAACCTCGACATTGAGCGCCCAA
CCTACACTAATTTGAACCGCCTCATTGGCCAGATAGTCTCTTCTATCACAGCCTCCTTGCGCTTTGATGGGGCCCTCAATGTGGACTTGACAGAATTCCA
GACCAATTTGGTGCCTTATCCTCGTATACACTTCCCTCTTGTCACGTACTCTCCTATAATTTCTGCCGAGAAAGCATACCATGAGCAGCTCTCTGTGTCT
GAAATCACAAATGCCTGTTTTGAACCATCTAACCAGATGGTAAAATGTGACCCACGACACGGCAAGTACATGGCCTGCTGTATGTTATATCGTGGAGATG
TTGTGCCAAAGGATGTGAATGCTGCAATTGCGGCTATTAAGACCAAACGTTCTATACAGTTTGTGGACTGGTGCCCAACAGGTTTTAAGGTGGGAATAAA
TTACCAGCCACCTACTGCGGTTCCAGGTGGTGATCTTGCCAAAGTTCAGCGCAGTCTGTATGCTCAGCAACACAACAGCTATTGCCGAAGCCTGGGCACG
TCTGGACCATAAGTTTGACCTTATGTATGCAAAACGTGCTTTTGTGCATTGGTATGTTGGTGAAGGCATGGAGGAGGGTGAATTTTCTGAGGCACGAGAG
GATCTTGCTGCTTTAGAAAAAGATTATGAGGAAGTGGGCACAGACTCTATTGAGGGAGAGGATGAAGGGGAGGAATATTAGTGCCATTCCATTGAAGTGC
ATTATACAGAATGTCATTCCTGACATAGCTGCTTGAAATAAAGGTTTTTATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAA
5' EST: CF786454 [+]taattaatcccccccccccccccgagttgctgtgttggcggagacccgtagcgcgtctatagtcaacggagccaaagaggggcgagtgtaggaagactga
aggttatctggtcctttatttagttctaccggtgcgcacacatctaatcagaaaatgcgtgagtgtatatctgtccatgttggccaggctggagttcaga
tcggtaatgcatgctgggaactttactgcctagaacatgggatccaaccagatggtcagatgcctagtgacaaaactattgggggtggagatgattcctt
taacactttctttagtgaaactggagcaggaaaacatgttcctagagcagtgtttgtagacctggaatctgcagtcatagatgaggttcgtaatgggaca
tataggcagctttttcaccctgagcagcttataactggtaaagaggatgctgccaataattatgcacgtgggcactacactgtaggaaaagagatcattg
acttggtgctggaaagagtacggaaactggctgaccaatgcacaggtctccagggttttctgattttccacagctttggaggaggaacaggatctggatt
tacatccttgcttatggagagactatctgtagattatggcaagaattccaagttggaatttgccatctatccagccccacaagtgtctacgcctgtggtt
ggaaccctataatttccattctttacacaacacactacactgggaacaactctgactggggccatttatgggggggataatggaggcccttttttggcac
t
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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