XB-CLONE-4332245
Clone Name: dazap2-HAR96
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Gene:
dazap2
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-96.
Xenopus tropicalis DAZ associated protein 2. Full length. Accession number AL644285.
To generate probe for in situ and mRNA for injection.
Clone previously called: TNeu027k13
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Sequence:
5' EST: AL644285 [+]
aatccccgggaagagacgtgaacccggggcaatgaataataaaggacagtaccctgctgccccagcctacccatcccagactccaaatcctcagtcagtg
tacccaccaaccatgcatctgccacaggctcctccttatacagatgcacctccagcttattctgagctctaccgatctgggtatgtgcatcaagcagcag
ctaatatggcccctctgtctgcagcttacccccccagcacatcaatgtatcttcctatggctcaggctatgcaagttgcaccaatggcctcatcagtacc
tatggcttattaccctattggtcctgtgtacccacctggctccacagtcattgttgaagggggctatgatgcaggcgctagatttggtgttggtagctcc
ccatctgttccaccacctccaccgggctgccccccgaatgcggctcagctggctgccatgcagggtgcaaatgtattggtcacacagaggaaaggcaatt
acttcatgggtggctcagatggtggctacaccatttggtgagatggaaccagaggcatgaaaacaggggttaaaaagcaaaaataaaac
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
RNA injection:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
sense |
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SP6 |
AscI |
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Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
ClaI |
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Suppliers:
Search for clone at:
EXRC