XB-CLONE-4280366
Clone Name: tubb2b-HAR52
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Gene:
tubb2b
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-52.
Xenopus tropicalis tubulin, beta 2B class IIb. Tneu143I02 - AL798377 - 5' present
Note this clone is chimeric. The 5' end of this clone is the full length tropicalis neural specific tubulin (~1.7kb). However a portion (about 1.1kb) of 18S sequence is fused to the 3' end of the tubulin clone (same orientation). Therefore this clone is NOT suitable for in situs.
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Sequence:
5' EST: AL798377 [+]
ccggccagacaccataggggtgtgcacaaaggggcaagtaacgcaacaagaaagggctacaacatccactgatttacaccgcttataagaccaataccaa
tcaatcgtaaccatgcgtgaaatcgtgcacatccatgctggccagtgcggtaaccaaattggagctaaattttgggaagtcatcagtgatgaacacggga
ttgatcctacaggcagttaccatggagacagtgatttgcaactagaaaggatcaacgtatattacaatgaagccacaggtgacaaatttgtaccccgtgc
catccttgtggatttggaaccaggcacaatggattctgtcagatctgggccattcgggcagattttcagaccagacaactttgtctttggtcagagtggt
gctggcaataactgggccaagggtcattacacggaaagagctgagctggtggactctgttctggatgtggtgagaaaagaagcagagagctgtgactgcc
tacaaagttttcagttgacccattctct
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC