XB-CLONE-4274312
Clone Name: twsg1-HAR91
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Gene:
twsg1
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-91.
Xenopus tropicalis twisted gastrulation homolog 1. Accession number AL634092. TGas012m12.
To generate probe for in situ.
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Sequence:
5' EST: AL634092 [+]
tttggtggccaagtctgaagccctctctcctacccattccagcagcagctctgctcctctgctctttctggattttgcctgttcgttgctgcaataaggc
tctgtgtgcaagcgatgtcagtaaatgtttattacaggagctgtgccattgtcggcccatggatggaaactgctcctgctgtaaggagtgtatgctgtgc
ctgggcacactctgggacgaatgctgcgactgcgttaatatgtgcaacccaagaaactacagcgatacacccccaacctctaaaagtacagtggaggaga
tttatcaatccattccatccctgttccgggctcttactgaggaagacacccaacttaactggaatattgtaaccttccctgtagtggaagagctttccca
tcatgagaacctggtctccttcctggaaactgtaaaccaaccacaagagcaaaacgtatcagtcgaggtctctcacagcaatgacaaagagcacatgtgc
acagtagtgtactttaatgagtgtatgtcaatccatcagtgcaaggtttcctgtgagtccatgggagcctcgaagtaccggtggttccacaatgcttgct
gtgaatgtgt
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
ClaI |
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Suppliers:
Search for clone at:
EXRC