XB-CLONE-4260109
Clone Name: pax2-HAR114
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Gene:
pax2
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-114. TNeu062i10.
Xenopus tropicalis paired box 2.
Accession number CR760264 - BamHI digest/T7 works for in situ. However BamHI digest does produce a second small band 600bp.
To generate probe for in situ.
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Sequence:
Full length sequence: CR760264 [+]
CTAGCACACACATCCCACCTTAAAGTGACAACTCCTGCCGGTCTCTCCATTTTAGGGATATTTTGCAGCCCCCCCCAAACAAATCACACACCCCTGGATT
CTTTTTTTCCTCTGCTTTTCGCCTCCTGCTTAACTTTGCGTTGAAGTCAGACAAGTCCAGCCAAAGACTTTGGAGGAAAGCAAACTCACCTTGCACCTTG
TGCTGCTGCTCGCCACTGGCTTTTTGCCTCGCTGCCCTACAAGCCTGCTCCAGGAGAGAGCTGTAGTTATGCCATGCACCTGCCGCCCTGAGATTTGCTC
CTGTGCCCGGGAAGGCTGCTGAAGGGAACTCGTTCCAAGCTTCCTGACTTGCCTCTGCCTTCGCCCCACGCTGCCCGCACTGCTGCCATTCCTGCCTCCA
GCTCCAGCAGCAGAGGGGGAGAGGGGAGTACAGCGCAGCTCAGACCCTCCACCTGATTTTCTTCAAGTCTTGAAGTTCAGTCCAGAGGCGAAACGGCGAC
TACAGCCGAATTCCTCCCCTTCATTTGCCTCCCCATGGATATGCACTGCAAGGCAGATCCCTTCTCGGCCATGCACCCAGGGCATGGGGGTGTGAACCAG
CTCGGGGGGGTATTTGTCAATGGCAGACCCCTACCTGATGTGGTGAGACAGAGGATAGTGGAACTGGCCCATCAAGGAGTGAGACCCTGTGATATTTCCA
GGCAGCTGAGGGTCAGCCATGGATGTGTGAGCAAGATCCTGGGCAGGTACTATGAGACGGGGAGCATTAAACCTGGAGTTATTGGCGGATCCAAACCCAA
GGTTGCTACTCCCAAGGTTGTCGACAAAATCGCTGAGTACAAGAGACAAAACCCTACAATGTTTGCTTGGGAAATCCGGGACAGGCTTTTGGCTGAAGGA
ATCTGTGACAATGACACCGTGCCCAGTGTGTCCTCCATAAACAGCAAACAGACTTAAAGCCGCAAATAAGCCAGCCATTCTCGCACGTGTAAAACTGGTC
AGGATTACGAGGCGTTTACGTAGGATTCTTCTCGTAACCCAAACTTTGATTGACAAACAGAAAACACTTTGTGAGGAACCCCTTTTCCTGCTTGTTCACT
GGCAATTACTGGCTTTAATGGATTAAAAAAAAAAAAAAAAAA
5' EST: AL673599 [+]ctagcacacacatcccaaccttaagtgacaactcctgccggtctctccattttagggatattttgcagccccccccaaacaaatacacacccctggattc
tttttttcctctgcttttcgcctcctgcttaactttgcgttgaagtcagacaagtccagccaaagactttggaggaaagcaaactcaccttgcaccttgt
gctgctgctcgccactggctttttgcctcgctgccctacaagcctgctccaggagagagctgtagttatgccatgcacctgccgccctgagatttgctcc
tgtgcccgggaagggctgctgaagggaactcgttccaagcttcctgacttgcctctgccttcgccccacgctgcccgcactgctgccattcctgcctcca
gctccagcagcagagggggagaggggagtacagcgcagctcagaccctccacctgattttcttcaagtcttgaagttcagtccagaggcgaaacggcgac
tacagccgaattcctccccttcatttgcctccccatggatatgcactgcaaggcagatcccttctcggccatgcacccagggcat
3' EST: BX686274 [+]gcttttttttttttttttttaatccattaaagccagtaattgccagtgaacaagcaggaaaaggggttcctcacaaagtgttttctgtttgtcaatcaaa
gtttgggttacgagaagaatcctacgtaaacgcctcgtaatcctgaccagttttacacgtgcgagaatggctggcttatttgcggctttaagtctgtttg
ctgtttatggaggacacactgggcacggtgtcattgtcacagattccttcagccaaaagcctgtcccggatttcccaagcaaacattgtagggttttgtc
tcttgtactcagcgattttgtcgacaaccttgggagtagcaaccttgggtttggatccgccaataactccaggtttaatgctccccgtctcatagtacct
gcccaggatcttgctcacacatccatggctgaccctcagctgcctggaaatatcacagggtctcactccttgatgggccagttccactatcctctgtctc
accacatcaggtaggggtctgccattgacaaatacccccccgagctggttcacacccccatgccctgggtgcatggccgagaagggatctgccttgcagt
gcatatccatggggaggcaaatgaaggggaggaattcggctgtagtcgccgtttcgcctctggactgaacttcaagacttgaagaaaatcanggtggagg
gtctgaaagctgcgctgnntactcccctctcccnncctctgctg
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
ClaI |
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Suppliers:
Search for clone at:
EXRC