XB-CLONE-4145380
Clone Name: intu-HAR71
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Gene:
intu
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-71. TGas012e01.
Xenopus tropicalis inturned planar cell polarity protein. Not full length. Accession number AL629224.
To generate probe for in situ.
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Sequence:
5' EST: AL629224 [+]
cactgacaatatttataacactatcaagttaacttcaggcccagaaaatctctcttccattacttatactttgaaactctacaaggggtctttatctctc
caacacacaaagaggtagaacagcttggtggctccattcatcctcagctgatcaagaatttccatcgctgctgtctgtcaatccattctgtgtttcagca
agctctaaatcaaaagaactccaaacagagtgattctgtggcctgcatggatgttgcagggtttggtcccattaaagaacatggagttttatttgagtgc
tctcctgagaattggaatgaccaaaagaaatccccgccaactatgtcatattgggttgtagggagactcttcatgcagccgcagcctcaggagctctatg
tttgttttcacgactccgttacagaaatttctgttgagctggcctttaagctctccttcgggatgcctttgtaaatggaatacagcttaaatagacctgt
tatgtgtgcagggaaaacaatctgtcccctatctgtatttagtacatattgtactagacttttatcattatcgcttattctaaaatgcacattttttatt
cgcttttctatttaatttttttaaccactgtgacctctgtgttattgcctgacacttaatatcagtgc
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
ClaI |
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Suppliers:
Search for clone at:
EXRC