XB-CLONE-4017569
Clone Name: sia1-HAR41
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Gene:
sia1
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-41.
Xenopus tropicalis siamois homeodomain 1. Tgas088L16 - AL960285 - 5' present.
To generate probe for in situ.
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Sequence:
Full length sequence: CR761649 [+]
CCCGGGGAGACAGACATGACCTGTGAGGCTGAAATGGAGCAGATTGTCTCCACTGCGCTGACTCTGCAAGAGGACTACATCAAATTCACCCCAAGGAACC
CCACCATGGCCTGCCACACTGAAATCATTGGGGTATTTCATGATATTCACCCCACTGTGGAGAAAAACAAGGAAGCCCACCGGGATAAATTGGTTCTACA
AGAGACTCTGGTGGAACTCTACTCCGTCTTGGGAATTCCACAGGAGCCCCAGGTCGGCAAAACCATGAAGTTTGAAGAACCAGAACAACATAGAGGATCA
GCTAGGTTTGACCCCCTAGTCAACAGCCTGCAGTTGAAAAGGCCTTTATACGAGGAGGAACGGAGGGAATGTAAGAGGCCGCTGGTTCAGGCAGATGAGG
CCGTGGCAGCCCCCAGTGCCAGATCCAGGAGGAGAACCATTTACTCCAAGGAGCAAACCCACTTCCTTCAGAATCAATTTGACCTCAACCCTTATCCGGA
CTTTGTCAACAGATGCCGCATTGCCAAGATAACCGCCATTCCTGAGCCCCGGATACAGGTTTGGTTCCAGAACAGAAGAGCCAGACACCTCCCAAGAGCG
GCCACGTCCCTGTCCCCCCAGGAAAGGAAACCCCCGGGTTCTGTAGGGCCCAGATCTTCCCTGTGCAGAGAAGCCCAACACCCAGAAGCTTGGAGTGAAG
TTCTGATCCCATCGGACTCACAGCCCCACCCAAACTGACTTCTACAATCAACTCAATGGCTTTTGTACTTCAATGGACTTTTATTATGGGGGGGTTTCTT
ATAAATAACTTTACTGGCTGCCGATTGGTGCATATGTTACAGGGGATGCTGACATTTATTTTCTATATTTTAGTATTTTATTTATTTTAATATCTTATAG
TAATAGACCCCGTTATTTATAGACATTATATTCTGTTCATTCTGTGCTTCATTATTATCTAAAGTTGTATTATTTATGGTTTTTTTAATTATTTGCCTTC
TGCTTCTGCCACTTTCCAGCTTTCAAATGGGGGTCGCTGACCCCGGCAGCCAAACCCTATTGCTCTGTGAGGCTCCAGTTTTATTGTTATTGTTACTTTT
TATACCTTTATTTTTCAACTTAGGCCCTTTCCTGTTTATATATCGGCCTCTCGTGCAAACCACTCCCTGGTTGCTAAGGTAATTTGCTTCCTAGCAACCA
GATAGCAGCTGAAACTCCATTCACTGAATAAAAAGTGAAATAACTAAAACACTACAAATAATAAAAACTAGTCAGATATTCACTTTCACACAGCAGACTG
TTGCCAGTTGATTGGTTGCTATGGGTTACTAGACTTCCATATATTTTAGATTTTTTTCTGTATCCACACAGGGCATTGCTTGAAACTGCAGATGTGGCAC
AATTATGTATTTAGCAATACAAGAAGATTAATATATAGTTGGACGTTTGGTTTTCAATAAAGATGAGATGTTATTTATAAAAAAAAAAAAAAAAAA
5' EST: AL960285 [+]cccggggagacagacatgacctgtgaggctgaaatggagcagattgtctccactgcgctgactctgcaagaggactacatcaaattcaccccaaggaacc
ccaccatggcctgccacactgaaatcattggggtatttcatgatattcaccccactgtggagaaaaacaaggaagcccaccgggataaattggttctaca
agagactctggtggaactctactccgtcttgggaattccacaggagccccaggtcggcaaaaccatgaagtttgaagaaccagaacaacatagaggatca
gctaggtttgaccccctagtcaacagcctgcagttgaaaaggcctttatacgaggaggaacggagggaatgtaagaggccgctggttcaggcagatgagg
ccgtggcagcccccagtgccagatccaggaggagaaccatttactccaaggagcaaacccacttccttcagaatcaatttgacctcaacccttatccgga
ctttgtcaacagatgccgcattgccaagataaccgccattcctgagccccggatacaggtttggttccagaacagaagagccagacacctcccaagagcg
gccacgtccctgtc
3' EST: BX752484 [+]cgcttttttttttttttttttataataacatctcatctttattgaaaaccaaccgtccaactatatattaatcttcttgtattgctaaatacataattgt
gccacatctgcagtttcaagcaatgccctgtgtggatacagaaaaaaatctaaaatatatggaagtctagtaacccatagcaaccaatcaactggcaaca
gtctgctgtgtgaaagtgaatatctgactagtttttattatttgtagtgttttagttatttcactttttattcagtgaatggagtttcagctgctatctg
gttgctaggaagcaaattaccttagcaaccagggagtggtttgcacgagaggccgatatataaacaggaaagggcctaagttgaaaaataaaggtataaa
aagtaacaataacaataaaactggagcctcacagagcaatagggtttggctgccggggtcagcgacccccatttgaaagctggaaagtggcagaagcaga
aggcaaataattaaaaaaaccataaataatacaactttagataataatgaagcacagaatgaacagaatataatgtctataaataacggggtctattact
ataagatattaaaataaataaaatactaaaatatagaaaataaatgtcagcatcccctgtaacatatgcaccaatcggcagccagtaaagttatttataa
gaaacccccccataataaaagtccattgaagtacaaaagccattgagttgattgtagaagtcagtttgggtggggctgtgagtccgatgggatcagaact
tcactccaagcttctgggtgttgggcttctctgcacagggaagatctgggccctacagaacccgggggt
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
ClaI |
Do not use Ecor1, BamH1 or HindIII |
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Suppliers:
Search for clone at:
EXRC