XB-CLONE-4010304
Clone Name: TGas098b15
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Gene:
bub1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: CT025409 [+]
CGGGGCCCTGGAATCGCCACCTTACTGCTCATCATAAAAATGGATCTACAGAGCCATGCACAGATGTTTGAAGCCCATATTCAGGGATACAAAGGCGATG
ATCCACTTGATCTTTGGGAAAGGTATGTACTTTGGGCAGAAGAAACTCTCCCCCCACAAGAGAAGCAGAACATTTTTTGTTTGCTTGAGCGTCTAGTTAG
AAACTTCATAGGAGACAAAAGATACAGCAATGATGAGCGATATCTGAAGTACTGCATCAGATTTGCTGAAACTATCAATGAGCCAGCACAATACTTTGAG
TACCTATATAACCAAGGCATCGGCCATCAGTCTGCCCCTCTGTACGTGACATGGGCTCAGTTGCTAGAGACACAAGGAGACCTGCAAGGTGCCAGTACTC
TGTATCAGAAGGCAATTCACAGCCATGCAAAACCTAAAGAAATCCTGGACCAGCACTTCAGAGCATTCCAGATCAGGGTCTCTCAAGCAAATATGCAAAA
TCATGGTGCCCTTGTGGAACCCCTCAGGAATTCTCAGATTTTAAATCAAATGAACTCTACTTCATCAAATGTGGATCAATTATCTCTTGCCAAATGTGAG
AGTTCTGCTCCTTCACAAATTCACACTTCTCAAGAATCTGCATGTGATGTTGATAGAAGTGGGAATAAATGGGTCACCATCTCCAAGTCAGCTGTGGTTC
CACAGCCTGCTAAATCTGTGGGTGTGGAAGTTAAACAGGTTCCTATGTACTGTAAGGATAAGCTGGTCTGTTCCGATTCTGAGCTTTCGTTTGAGGAATT
CCGAGCAAATATATACAAAAAGAAATGTGAGCAGCGCAAAAAGATGCAGCAGTGGGAGGAAGAAGAAAGGAAATACAAGAAGGTTAAGGAGGAAGCTTCT
CTCCAGGAACAACTCTTAAAGCAGAAGATGGAGCAGCTGAGCAGCCTGCTGAATGTCCAGGGAAGGCAGGATGTGCTGCCCCAAAGCACCGCTAGGCAAT
TGCCTGAGGTCCCCCAGACAATAACTAGCAATGTTTCTATAGCTAGTGGGTTGAAAATTTCAAATCTTCCAGAGGCAGAAACGCAAGCATCCAGTGTAAA
AGATGATCCAATAATTTTGCCTAGCTCTCATGTAGCATGTCCTTCTGTGCCACAAATGGCTCCTTTTGGGAGAAATGAGTGCAGCCCTGCTCCTGAAGAG
TGCATGCTTCCTCCAATGGATCAGAACTCTATTCTTTGTAAAGCAGCATCAAATGTTCCAAGAGCTCATATTGCTGAACAAAGTATCCTGAGCAAACAGC
AATCTAATCTTGATAGATCAACAGCGACTGTTCTTGAGATGTCTAAGCAAATATGTCCAGACACAAGTACACAAGGTCTCAGATTTCAGCCTGGAAAAAA
GGAAGCTGCTGCACCTGGAAACTTATCTGGATATTTAGCAAACACATCGCATGTAACTCCCAACACTTCTCTAGGCTTGGTGCAGGCCACTCCATCCAAA
GTACTGCCTTCACCCACTGTCAATACTAAGGAAGCGCTTGGTTTCATAATGGACATCTTCCAAACATCCACGCTCCCAGATAATGTGGAAGAGGAGGAAG
AAACGCAAGATGAAAATGATCAGGAGTTTGAAGCCTTTTGCAGAAATGACTACAAAACTAATTCTAACACTGTTGGTTTCCTGGGACTGCAGAATGTTGC
CCCTGCATTGCCATCTGCCTTCTGCATCTTTGAAGACAATGTTAACAAAGTGAATGACTTACAGAGCAAACCAGTAGAAGTAAAAACACTCCGCGAACGC
CCTGTGCTGAGGCCTCCTTTGAAATCTAATGAGGAGGTCAAGGCAGCAGAGTCCTTGGTAGATGACTGCACAGTGTGGGCTGTGCGATGCAACAAAACTC
TAGCCCCCAGTCCAAACAGCACAGGTGATTTTGCTCTGGCGGCCCGACTTGCATCTACTCCTGCCAACAAGCAGACAGAGTCAACATGGCAGACCGTGGA
GGATAAAGAGAATGTAATTGCTGACAGTGTTGTGCATACTGTTTTTGACTTTGCTGAGGATAAAGTAATTCAAGTCTCCAAATCCCGTAAGCTAAGTCCT
ATACAAGAACAAAGCCCAGAGCATTCCAAAATCCATGGGCCAGTACAGTCTCCTTCATGCACCATTCTGCCTGCTGAATCTCCAGCAGCTAAGTTTCTGT
CTGAAGATGTGGAGCAGACTGGCCAGAAACTGGCAGCATGCAAACTGTCAGACACATTGCACCAACCTGTACTGGGAAGCCTGGAAGATCCCTGGGGGAT
AACAACACAAATGCTGGATGCTCATGAAGAAGAACCAACAGAAACAATTGTTAATGCTCCACCTGAACTGGTTATTATTGAAAATGCATGGGATGATAAA
CTGATCGACCGTCTCCTGTCTGAACTGCCTAAATCACTTAGTTCCCACGAAAATTACTATCAGTGGCACACAATGGTTCCTGTTTTGAAACCCAAGATGG
AAGTGAAGCTGGGATCCAGTTCTTTTTATATTGATTATCTGCTTGGGGAAGGAGCATTTGCGAATGTCTATCAGGCATCATTGTTGGGTAATCAGAAAGT
TATATTAAAGGTTCAAAAGCCAGCCAAGCCATGGGAGTTCTACATTGGAACACAGATAAGAGAGAGGATAAAGCCAGAATTAAGGCATCTATTTATAGGC
TTCCAGGCTGCACATCTGTTTGATAATGGAAGTGTGCTGGTTGGTGATCTGTACAACTATGGCTCACTTCTGAATGCCATCAATTTGTACAAGAAGCTGA
GTGAGAAAGTCATGCCAGCACCACTAGTCATGTACTTTGCCATCAACATTCTGTACATGGTGGAACAGCTCCATGACATTGGCATTATCCATGGAGACAT
CAAGCCTGATAACTTTGCAATTGGTGAAAGGTTTCTGGAGAACGAATCCTGCAGCCTTGATTTCGTTTCTCATGGTCTTGCACTGATTGATCTGGGTCAG
AGCATAGATATGACTCTCTTCCCTAAAGGAACTGCTTTTACTGGAAAGTGTGAGACTTCTTGCTTTCAGTGCACTGAAATGCTGACTAAAAAGCCATGGA
ACTATCAGACTGACTACTTTGGAGTGGCTGGGACAGTTTACTGTATGATGTTTGGGAATTATATGAAAGTGAAGAACGAGCAAGGAGTCTGGAAACCAGA
TGGCACTTTTAAAAGATACCAACATGGGGGGGTTTGGATGGAGTTTTTCCACACATTATTGAACGTCCCTGATTGTCACAGTCCTTCCCCCCTCCGAGCC
CTTCGGAAAAAGCTTATGTCCACCTTCACGCTGTACACCAACAAGATCAAATCGTTCCGCAACAGACTTATCATCCTGCTTTTAGAGAACAAGCCTTCCC
GTAAATAGACTTTTAAATCCAAGCTCGATACTTTGTGTAGGTTTTATGGAAATGTATGGAAGTACCTACTCAATTGGGTGGCCTTTGGAATAAGAGGTTG
AAAAACAGAATATTTTCCAGTTCTTCAGGATTAAATAAAGATAAATCTGCAGTAATTCCTGCAAGAAGGAAAGGCCTAATACAATGCAATACATTCAAAC
GGTCCAGACTTCGTAGTAGTAGTGTGTGCAAACGGCACACTTTTTGGATTTGGATTCCAGCCAATCTGTTCACAGTTTGTATTGTATTCCTAGTTAAGTA
GCGGGCTCGCACTTGCCTTGATTAGATCCTGCCTGTGTATTAGGCAGAAAGTATTGTATGTGGTGCATCCATATTTTTATTTTGTAATATTCTGTACATA
TTTCGTTTTTTGCGGGGATAAAAAAAAAAAAAAAAAA
5' EST: AL958559 [+]cggggccctggaatcgccaccttactgctcatcataaaaatggatctacagagccatgcacagatgtttgaagcccatattcagggatacaaaggcgatg
atccacttgatctttgggaaaggtatgtactttgggcagaagaaactctccccccacaagagaagcagaacattttttgtttgcttgagcgtctagttag
aaacttcataggagacaaaagatacagcaatgatgagcgatatctgaagtactgcatcagatttgctgaaactatcaatgagccagcacaatactttgag
tacctatataaccaaggcatcggccatcagtctgcccctctgtacgtgacatgggctcagttgctagagacacaaggagacctgcaaggtgccagtactc
tgtatcagaaggcaattcacagccatgcaaaacctaaagaaatcctggaccagcacttcagagcattccagatcagggtctctcaagcaaatatgcaaaa
tcatggtgcccttgtggaacccctcaggaattctcagattttaaatcaaatgaactctacttcatcaaatgtggatcaattatctcttgccaaatgtgag
agttctgctccttcacaaattcacacttctcaagaatctgcatgtgatg
3' EST: BX756122 [+]cgctttttttttttttttttttccccgcaaaaaacgaaatatgtacagaatattacaaaataaaaatatggatgcaccacatacaatactttctgcctaa
tacacaggcaggatctaatcaaggcaagtgcgagcccgctacttaactaggaatacaatacaaactgtgaacagattggctggaatccaaatccaaaaag
tgtgccgtttgcacacactactactacgaagtctggaccgtttgaatgtattgcattgtattaggcctttccttcttgcaggaattactgcagatttatc
tttatttaatcctgaagaactggaaaatattctgtttttcaacctcttattccaaaggccacccaattgagtaggtacttccatacatttccataaaacc
tacacaaagtatcgagcttggatttaaaagtctatttacgggaaggcttgttctctaaaagcaggatgataagtctgttgcggaacgatttgatcttgtt
ggtgtacagcgtgaaggtggacataagctttttccgaagggctcggaggggggaaggactgtgacaatcagggacgttcaataatgtgtggaaaaactcc
atccaaacccccccatgttggtatcttttaaaagtgccatctggtttccagactccttgctcgttcttcactttcatataattcccaaacatcatacagt
aaactgtcccagccactccaaagtagtcagtctgatagttccatggctttttagtcagcatttcagtgcactgaaagcaagaagtctcacactttccagt
aaaagcagttcctttagggaagagagtcatatctatgctctgacccagatcaatcagtgcaagaccatgagaaacgaaatcaaggctgc
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
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