XB-CLONE-4006403
Clone Name: dkk1-HAR2
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Gene:
dkk1
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-2.
Full Lenght.
Clone previously called: TGas131c10
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Sequence:
Full length sequence: CR761605 [+]
CGCAGCAGTCACAACCGGCGCTGCACCCAAGCCTTCAGCAGCACCCAAGCACCCTAGCCATCAGCAGCACCCAAGTCTTCAGCAGCACCCAAGGGACTTA
TCCCCGGAGGACTTATCCTGCCGTTATTGGTTACTGGGACAGAGGAATGGACGAGATGTTCCTGATGCTTCTTTTTGCCTTGGGGGGGTTTATCTTGGAT
GGGGCACTTGGCTTTGTCATGATGACCAACTCCAACTCCATCAAGAACGTCCCAGCGGCCCCGGCTGGGCAACCCATTGGCTACTACCCTGTCAGTGTCA
GTCCGGACTCCTTATATGATATTGCCAACAAATACCAACCTTTGGATGCCTACCCGCTCTACGGTTGCACAGAAGATGACGACTGTGCCCTGGATGAGTT
CTGCCACAGTTCCAGAAATGGCAACTCCCTGGTCTGCTTGGCATGCCGGAAACGCCGCAAGCGCTGCCTGAGGGACGCAATGTGCTGCACCGGCAACTAC
TGTAGCAACGGAATATGTGTCCCCGTGGAGCAAGATCAAGAGCGCTTCCAGCAGCAGGGATACCTGGAGGAAACCATTCTGGAAAACTATAACGCCGATC
ACGCAACAATGGATACGCATTCAAAATTAACCACGTCCCCTTCGGGAATGCAGCCCTTTAAAGGTCGTGATGGTGACGTCTGCCTCAGATCAACCGACTG
CGCCCCAGGTCTATGCTGCGCCCGTCACTTCTGGTCGAAGATCTGCAAGCCGGTGCTTGAGGAAGGCCAAGTGTGCACCAAGCACAGGAGGAAAGGCTCC
CACGGGCTGGAGATCTTCCAGCGTTGCCACTGCGGCGCCGGACTCTCCTGTCGGTTACAGAAGGGAGAGTTTACGACCGTCCCTAAGACCTCGAGACTTC
ACACTTGCCAGAGACACTAAGTGAGGCCTGCAGAGCCTGAAGGACCTTCTCTAAATTAAGCTAATAAGGACATTGGCCGCTAATAAGGACATTGGCCGCT
AATAAGGACATTGGTCGCTCTGCCTTTGGGGGCGTATCAGTTTACATGAAGTGCTCTGGTCCCTCCCCCCCCCGAGCCCAGAGCTGCACAACTTGTTTCT
TTTTTATAGGAACTTCTTATTAATTCCTAATTACAGTAAATTACTGTGTTGTAAATTGGTTAATAATGATGAACAGCTGTAAATATCTGTGTATAGAAAA
TGTACATGATCCTCTCTATTGTAACCGGCCGCCTTGTACATTCCGACTCGCTCTTCCCTGTTTATATATAAATATATCTATATAAATATTATGTAGATTT
TAACGTCTAGTACGTCTGTATTTCTAATTGAAATAAAACATTTCCAAACCGAAAAAAAAAAAAAAAAAA
5' EST: AL969277 [+]cgcacagtcacaaccggcgctgcacccacgccttcagcagcacccaagcaccctagccatcagcagcacccccgtcttcagcagcacccaagggacttat
ccccggaggacttatcctgccgttattggttactgggacagaggaatggacgagatgttcctgatgcttctttttgccttgggggggtttatcttggatg
gggcacttggctttgtcatgatgaccaactccaactccatcaagaacgtcccagcggccccggctgggcaacccattggctactaccctgtcagtgtcag
tccggactccttatatgatattgccaacaaataccaacctttggatgcctacccgctctacggttgcacagaagatgacgactgtgccctggatgagttc
tgccacagttccagaaatggcaactccctggtctgcttggcatgccggaaacgccgcaagcgctgcctgagggacgcaatgtgctgcaccggcaactact
gtagcaacggaatatgtgtccccgtggagcaagatcaagagcgcttccagcagcagggatacctggaggaaaccattctggaaaactataacgccgatca
cgcaacaatggatacgcattcaaaattaaccacgtccccttcgggaatgcagccctttaaaggtcgtgatggtgacgtctgcctcagatcaaccgactgc
gccccaggtctatgctgcgcccgtcacttctggtcgaagatctgccagccggtgcttgaggaaggccaagtgtgcaccaagcacaggaggaaaggctccc
acgggctggagatcttccagcgttgccactgcggcgccggactctcc
3' EST: BX758076 [+]cgcttttttttttttttttttcggtttggaaatgttttatttcaattagaaatacagacgtactagacgttaaaatctacataatatttatatagatata
tttatatataaacagggaagagcgagtcggaatgtacaaggcggccggttacaatagagaggatcatgtacattttctatacacagatatttacagctgt
tcatcattattaaccaatttacaacacagtaatttactgtaattaggaattaataagaagttcctataaaaaagaaacaagttgtgcagctctgggctcg
ggggggggagggaccagagcacttcatgtaaactgatacgcccccaaaggcagagcgaccaatgtccttattagcggccaatgtccttattagcggccaa
tgtccttattagcttaatttagagaaggtccttcaggctctgcaggcctcacttagtgtctctggcaagtgtgaagtctcgaggtcttagggacggtcgt
aaactctcccttctgtaaccgacaggagagtccggcgccgcagtggcaacgctggaagatctccagcccgtgggagcctttcctcctgtgcttggtgcac
acttggccttcctcaagcaccggcttgcagatcttcgaccagaagtgacgggcgcagcatagacctggggcgcagtcggttgatctgaggcagacgtcac
catcacgacctttaaagggctgcattcccgaaggggacgtggttaattttgaatgcgtatccattgttgcgtgatcggcgttatagttttccagaatggt
ttcctccaggtatccctgctgctggaagcgctcttgatcttgctccacggggacacatattccgttgctacagtagttgccggtgcagcacattgcgtcc
ctcaggcagcgcttgcggcgtttccggca
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
RNA injection:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
sense |
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SP6 |
AscI |
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Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
EcoRI |
BamHI and HindIII can also be used. |
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Suppliers:
Search for clone at:
EXRC