XB-CLONE-3992793
Clone Name: rps3a/gdf3-HAR69
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Gene:
gdf3
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rps3a
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-69. TGas141f11.
Xenopus tropicalis growth differentiation factor 3. Not full length.
To generate probe for in situ.
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Sequence:
Full length sequence: CR855767 [+]
CCCGGGGGGAGCAACTGACCCTAGGCCAACTGGAAATTAAGTTCAAGCACAACTCGTATTATGGACAACAGTTCCATCTGCGCCTCTACCGCACCCTTCA
GCTATCTCTAAAGGGTATGAGAGAGAGCAAGATGAACAGGAAGCTTCTAGTGTCACAGTCTTTCCGGCTTCTTCATAAGTCCCTCTATTTTAACCTGACC
AAGGTGGCAAAGGACTGGAAAACCCCTGAGAAGAATATGGGACTGTTATTGGAGATATATGCTAGCAGTAAACTTGCAGGAGACAACAGATCATTTGCAG
TGTGTGAACCAATCCAGTCTTTCATTTACACTTCTCTGCTCACTGTGTCTCTAGACCCATCCAGCTGCAAAACTCCGCGAGCCAAGAGAAGTACTCATTC
ATCGCCTCCAACCCCAAGTAATATCTGCAAAAAGAGGCGCCTGTACATTGACTTCAAGGATGTTGGATGGCAGAACTGGGTGATTGCACCCCGTGGTTAC
ATGGCCAACTACTGCTATGGAGAGTGCCCCTATCCACTGACAGAAATGCTCAGGGGCACAAATCATGCTGTTTTGCAGACTCTGGTGCATTCTGTAGAAC
CAGAAAGCACCCCTTTGCCTTGCTGTGCCCCCACTAAGCTGTCTCCTATCTCCATGCTATATTATGACAACAATGACAATGTGGTACTGAGGCACTATGA
AGATATGGTAGTAGATGAATGTGGTTGCAAGTGAGTTTGGCTAGGACTACTCTTCTCATTAAACTTATTGTCTAAGGGACTGCTGGCAACTTAATCATGG
AGTGATTCTCCTTGTGTGACAGTGGCTTTGACCATTAGTGTTCAGCTACAATTCCCCCCATGAAGCAAGATAGGCTTCTGTTAGCTAGAAATTGTAACAT
TCGTACCAGGAAATGTATTCCCATAAAAACAGAGACTATATTTTTTGTAAAACCTGTTTCTCCCCAATAACATTAATATGCACAAATTTTATGCATTGTT
TTTAGCTAAAATTCAGATTCTTTGGCATATGCTAGGCACAGGAAGCAAAACAAAAGTTGCCCTTTAGGTGGATGGGCCACTTTCATTTGTGACTTTTAAC
AGAGCTACAAGATGAAATGCTTTGCAAAATGACTACTGCTCTCAATTTTAGCAAAAAACAATGCATAAAATTCAGATTCTTTGGCATATGCTAGGCACAG
GAAGCAAAACAAAAGTTGCCCTTTAGGTGGATGGGCCACTTTCATTTGTGACTTTTAACAGAGCTACAAGATGAAATGCTTTGCAAAATGACTACTGCTC
TCAATTTCTTTATGTATGTATATATTGGGCATATGGCTAGGTTGGTGCCCAAGAACTGGCCTGCACTTGTTGCTTTGCTCTTGAACTGTTAAGACTAATG
TTTAAATACAGTTGATTGTTGTAAATAAAATGTGATTTAACATTAAAAAAAAAAAAAAAAAA
5' EST: AL966390 [+]gagagcaagatgaacaggaagcttctagtgtcacagtcttttccggctttcttcataagtcccctctatttttaacctgaccaaggtggcaaaaggactt
ggaaaacccctgagaagaatatgggactgttattggagatatatgctagcagtaaacttgcangagacaacagatcatttgcagtgtgtgaaccaatcca
gtctttcatttacacttctctgctcactgtgtctctagaccatccagctgcaaactccgcgagcaagagaatactcattctcgcctccacccaagtatat
ctgcaaaagaagcgcctgtacttgacttcaggatgttggatggcagaactgggtga
3' EST: BX764956 [+]gcttttttttttttttttttttttttttttttttaacaatgaagttgttttatttccacattaaaaaaaaataaaataggatccgttcccaccaggggga
gcaggatataaatatgtgccaaaaatgtgcagccgttccccgtgccgctccatgtgtcgcccgtcgctgggtgctgggtactcggggggggtacaataca
aagtcatatgattgggggacaaagtggggacactgaaagcccaaatccggtgccaaagcaaaacgatccccccggtc
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC