XB-CLONE-3985224
Clone Name: TEgg045n18
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Gene:
spast
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: CR760974 [+]
GCGGAGCTGTAGCTCACTGTTCCCGGTGTTATGGCTGTGGGCCCCGTGAATGAATTCTCCGGGCGGAAGAAACGACAAGAAGAAACCGGTGACCCCAGCG
GCGGAGACTGGTCCCGGCCCGCCGACGCCTCCTCCTCCTCCAGCAGAGACGCAGGTCCTGCTCGCCCCGCCCTCACTGCATAAGCGGAACCTTTACCTGT
TCTCCTACCCCCTGCTCGCCGCTTTCTCCCTGCTACGCTTCCTGGCTTTTCAGCTGGGGCTGCTGTTCGTTTGGTTTTGTGAGCGGCTTTCCCGTAGGGT
CATGGCGGACAAAGGGAGTACGGCGGCCCGCACAGCAGCGGCTCCAGCTCAGGACAGGCCCCAGGAGCCTGAGGTAGTGAGGAGCTATCACCAGCAGGCC
TTCCAGTACATCTCTATGGCGCTGCGCATCGACGAGGAGGAGAAAGATCAAAAGGAGCAAGCCATTCAGTGGTACAAGAAGGGTATTGAGGAACTGGAGA
AAGGAATAGCTGTAACTATAACTGGCAAAGGTGAACAATATGACAGAGCCCGTCGTTTACAAGCTAAAATGTCAACAAACTTGTTAATGGCAAAGGATCG
CTTACAGCTACTAGAAAAAGGGGCTGTTCCCAAAAAAAAAGATCCACCATCGATTTCAAGTAACTCTTATTCTCGTGTAAAGGCTGCTCCTAAGAGCGGG
TCATTGGGCAACAGAATACCTAACTGTACTGGTGTATCATCTTCAGCTAGGCAAGCTGGTCCAAATGCACCTTCTAACAGGGGAGCGGCAGGAAAGAACA
ACACAAGAACAAATAAACCAACAACACCCACTACTGCAGTACGGAAAAAAGACATGAAAAATTTAAGAAATGTAGATAGTAATCTTGCAAACTTGATTTT
GAATGAAATTGTGGATAGTGGTCCTACTGTCAAGTTTGCTGACATTGCTGGACAAGATCTAGCCAAGCAAGCCTTGCAGGAGATAGTTATTCTTCCTTCT
ATACGACCCGAGTTATTTACTGGGCTAAGAGCGCCTGCACGAGGATTGCTGCTGTTTGGTCCTCCAGGGAATGGAAAGACTATGCTGGCAAAAGCTGTTG
CTGCTGAATCAAATGCAACATTCTTCAACATAAGTGCAGCGAGTCTGACTTCAAAATATGTGGGAGAAGGTGAAAAGCTGGTGCGTGCACTCTTTTCTGT
GGCTAGAGAATTGCAACCTTCCATTATATTTATAGATGAGGTTGATAGTCTTTTATGTGAAAGAAGAGAAGGGGAGCATGATGCAAGCAGAAGACTGAAA
ACTGAATTTCTAATAGAGTTTGATGGTGTACAATCTGGGGGAGATGACAGAGTTCTTGTTATGGGAGCAACTAACAGACCACAGGAACTTGATGATGCAG
TTCTCAGGAGATTCACCAAGAGGGTTTATGTATCTTTACCAAATGAAGAGACTAGACTCTTACTTCTCAAAAATCTTCTGAGTAAACAAGGTAACCCACT
TAATGAGAAGGAACTGACTCAGTTATCTAGGCTGACAGAAGGATACTCTGGGAGTGATATAACAGCTTTGGCAAAAGATGCTGCTTTGGGACCAATCAGA
GAGTTAAAACCTGAACAAGTCAAAAATATGGCTGCTAGTGAGATGAGAAATATAAAATATTCAGATTTTCTAAGTTCATTAAAGAAGATAAAGTGCAGTG
TGAGCCCTTCAACCTTGGAATCTTATATAAGGTGGAATAAGGAATTTGGCGATACTACAGTTTGAAAGACAGACCATCTTATTCAGGGATTAAGCGATGC
TGGATGAGATTGTTACGCAGAAATTCACCGCCAAGAAACTTTGCGTATCTTTTGCTTTCAAAACAAAGAAAACTTTAAAAAAAAGCAAACCTCAGATGTG
TGCACTAAACTTCTAGAAACTGAATGAGCGAGCAGAAGAAAAGTACTTTATACAAAAAGCTGGCGATATTAACAACTCATGGGGCACTAACTGTAACTTT
GAGCGCTTATAACTGCAATTACAGCCTTTGGTTGAAAAGGAACCTTCACACTGTGTGCAAAATTAAGGCTACTCGGAGGAAATGTGGCATATTGGTACTG
CTTTCTACCAGTTTGGCTGTGGGAAGACCTGTGCCTTTGCTTTATGTTTCAGGCTTATTTGTGCCTGCCCCTTGTTTGTAATAAATTAATGTTAGACACT
CCTTCATACGTACTAATTGCAACTTTTTGTAAATTTATAGAGTTTCTTTAGGGTTGGGTTGGTCAGGGGAAAAGTTGACATGCTTAACGTTAAAGAGTTT
TTTATTTCATTATGTGCCAAATGCAAAGACAGAATTGGTTAAAAACGGACTGCCTTACTTGTGCAATAACACCAGTGGTATGGTTTATTAAGGTGACTGA
AATCATTTTAGTTTTTCCCTGTAAATGTACTACCAAGAGTAATTTATAGAGTAACCTGGCATAATGATCAAGCACAACTTGCATGCACAGCGGGTCATTT
TGTTTCTTTTTTTTTTTCCTTTATTGATGGTGTTAGATCAGTTCTCCTATTAAAAAGCAAGGGTTGGTCAACTGTGAAATAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA
5' EST: AL856941 [+]ggcggagctgtagctcactgttcccggtgttatggctgtgggccccgtgaatgaattctccgggcggaagaaacgacaagaagaaaccggtgaccccagc
ggcggagactggtcccggcccgccgacgcctcctcctcctccagcagagacgcaggtcctgctcgccccgccctcactgcataagcggaacctttacctg
ttctcctaccccctgctcgccgctttctccctgctacgcttcctggcttttcagctggggctgctgttcgtttg
3' EST: BX772802 [+]gcttttttttttttttttttttttttttttatttcacagttgaccaaccctcgttttttaataggagaactgatctaacaccatcaataaaggaaaaaaa
aaaagaaacaaaatgacccgctgtgcatgcaagttgtgcttgatcattatgccaggttactctataaattactcttggtagtacatttacagggaaaaac
taaaatgatttcagtcaccttaataaaccataccactggtgttattgcacaagtaaggcagtccgtttttaaccaattctgtctttgcatttggcacata
atgaaataaaaaactctttaacgttaagcatgtcaacttttcccctgaccaacccaaccctaaagaaactctataaatttacaaaaagttgcaattagta
cgtatgaaggagtgtctaacattaatttattacaaacaaggggcaggcacaaataagcctgaaacataaagcaaaggcacaggtcttcccacagccaaac
tggtagaaagcagtaccaatatgccacatttcctccgagtagccttaattttgcacacagtgtgaaggttccttttcaaccaaaggctgtaattgcagtt
ataagcgctcaaagttacagttagtgccccatgagttgttaatatcgccagctttttgtataaagtacttttcttctgctcgctcattcagtttctagaa
gtttagtgcacacatctgaggtttgcttttttttaaagttttctttgttttgaaagcaaaagatacgcaaagtttcttggcggtgaatttctgcgtaaca
atctcatccagcatcgcttaatccctgaataagatggtctgtctttcaaactgtagtatcgccaaattccttattccaccttata
Library:
Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from egg. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' endAnatomy: egg | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
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