XB-CLONE-3956068
Clone Name: xthr10F21
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN095853 [+]
ggggtcaccctgaagtttgctccgtctctaaagcaggtctagcccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattc
ctctgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacacca
agtggcttcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcct
atgaggtgttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaacct
taagggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgt
ggggagcgtcgtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatg
cagagcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatg
3' EST: CN095852 [+]atcatgggggggtttaaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatcttctcttttatgttgatt
ttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccatttt
ttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgt
gctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtccactt
cagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcc
cagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggaca
taacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagcgtctgaagacttctttca
tgtttcctc
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: