XB-CLONE-3955944
Clone Name: xthr10N15
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Gene:
tmem182
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN095934 [+]
aggggggattcctcattatctgtgctgctcccttcacaaaccacagaatttataaagcaggagggggcctattcataacatctggaattctctttgcact
ggtggttgtaatgcacgtcttttgggtacagagcgtatctgatatcaagggttacacggacaccagacggcaagattgttcccaattcaccgtgtatgtt
agctttggctggtcattcatgttggctccatttggaatatttttctgtttatttgcaggaatgctttttctgctagttggacatactatacatgtacata
ccaagtaacacaaaaaaaacagaaataacttcccagtaatttcttttctatatctgtcaatccgtaataatagggttttgtaaacattttaatcttttat
tatgtcaatgtcagaccaaatcatagaattagaatgtgtttattctgtatttaaatgtactatataagcaaacacataacttatttttttctgttctctt
caatgtgcaaaatacacaacatccttggcagagaaagagaatgggttgtaagtgtaattttatctgctcatgggctgcttgctggtatctgtcagttttg
ttcctgccataacattctagtaaatgtgcactccaggctgaaaatagattacagttgttcatttggtatc
3' EST: CN095933 [+]tgttaaaagataacacagggcactgaagtccgaagtactataaacagaagtagtaatggtagtgtatcaagaatgtgccattacaggaaacttataagac
atataacagagtgctaaagacaatccaacaggaaagcaatcttcgtgtgaatcatcctttgctggcgctgataccaaatgaacaactgtaatctattttc
agcctggagtgcacatttactagaatgttatggcaggaacaaaactgacagataccagcaagcagcccatgagcagataaaattacacttacaacccatt
ctctttctctgccaaggatgttgtgtattttgcacattgaagagaacagaaaaaaataagttatgtgtttgcttatatagtacatttaaatacagaataa
acacattctaattctatgatttggtctgacattgacataataaaagattaaaatgtttacaaaaccctattattacggattgacagatatagaaaagaaa
ttactgggaagttatttctgttttttttgtgttacttggtatgtacatgtatagtatgtccaactagcagaaaaagcattcctgcaaataaacagaaaaa
tattccaaatggagccaacatgaatgaccagccaaagctaacatacacggtgaattgggaacaatcttgccgtctggtgtccgtgtaacccttgatatca
gatacgctctgtacccaaa
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: