XB-CLONE-3948019
Clone Name: xthr18B16
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Gene:
etfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN101078 [+]
gctgcctgagtcgcttgctgaaggtgtcagaggccgggtcacctttgtgtctgtccaagagttcagcatgtaccgagcactgagtaccgggaggcagaag
gggactccatctctgcgcaggttccctagcgccctggttattgcagagcatgacacccaacaggtgacaccggtcactctcagcgccataactgcagcca
agcgacttggggcagatgtttcctgtttggtggcagggaccaactgcagtaaggtagcagaggagctggcaaaggtacagggagtgtcaaaactgttcgt
agcacagcatgaatcatacaagggatttttaccagaggagctgactccattggttttggaagcacagaagaagttcaatttcactcacatttgtgccgga
gcatcagcgtttggaaagagtcttctaccaagaattgctgcaaagcttgatgtggctcctgtatctgatgtcattgagattaaatctccagatacatttg
tgaggaccatctatgctggtaatgctttatgtactgtgaaatgtgaggagtctgtcaaggtgttttctataagagggacatcctttgaggctggctgaaa
catcaggaggaagtgc
3' EST: CN101077 [+]tttgaagcagcagaagtattgctcggaaccatcagtcgcagcttatacaaaaataatttgccaacattccattactttgcagggatcagtgtgctgtggg
tgagtgcggcacttcagtctatggctggatcactgatgattaaacaagcatctcttattcatccaagtgcagaagtcactttttcttaagtatttcagtc
atttctggcactgccttgaacaagtctgccaccaaaccatagtcagccacctggaagattggtgcctcaggatccttgtttatagcaacaatagtcttgc
tatctttcatcccagcaaggtgctgaatagctccagagattccaacagctatgtacagttcaggagcaacaattttgcctgtctgtcccacctgcatgtc
attgggtacaaagccagcatccacagcagcacgggaagcgccaactgcagcattcagctgatctgccagatcatataacagcttgaaattttctccactc
tttaaacctcggcctccagatacaacaaccttggcgctagtcagctccggacggtcgctttttgtcaaactctgatgaagccactcagatagtccagctg
gtgcagatggagccattgtgtctgagcttgcacttcctcctgatgtttcagcagcctcaaaggatgtccctcttatagaaaacaccttgacagactcctc
acatttcacagtacataaagcattaccagcatagatggtcctcactaatgtatctggagatttaat
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: