XB-CLONE-3947735
Clone Name: xthr19C11
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Gene:
tuba4b
,
tuba5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN101260 [+]
gagttgctgtgttggcggagacccgtagcgcgtctatagtcaacggagccaaagagggcgagtgtaggaagactgaaggttatctggtcttttatttact
tctaccggtgcgcacacatctaatcagaaaatgcgtgagtgtatatctgtccatgttggccaggctggagttcagatcggtaatgcatgctgggaacttt
actgcctagaacatgggatccaaccagatggtcagatgcctagtgacaaaactattgggggtggagatgattcctttaacactttctttagtgaaactgg
agcaggaaaacatgttcctagagcagtgtttgtagacctggaatctgcagtcatagatgaggttcgtaatgggacatataggcagctttttcaccctgag
cagcttataactggtaaagaggatgctgccaataattatgcacgtgggcactacactgtaggaaaagagatcattgacttggtgctggaaagagtacgga
aactggctgaccaatgcacaggtctccagggttttctgattttccacagctttggaggaggaacaggatctggatttacatccttgcttatggagagact
atctgtagattatggcaagaagtccaagttgga
3' EST: CN101259 [+]gagaggaagctggcaggatatacttttctagcattgggttcttacaggttgggaggggcagtgttaaactaggccaactggcaccttccaatctttggaa
taatacgatgatggataaagggcaattaatactcctctccctcatcttcatcttcataggagtcaatgccgacctcttcgtaatccttctccagggcggc
catatcctcgcgggcctcagagaactccccttcctccattccctcccctacataccagtgcacaaaggcacgcttggcatacataaggtcaaacttgtga
tccaggcgagcccaggcctcagcaatggcggtggtgttgctcaacatgcacacagctcgctgtaccttggccagatccccacccggaaccgctgttgggg
gctggtagttgataccaaccttgaaaccagttgggcaccagtccacaaactggatggtacgtttggtcttgatagcagcaatagcagcattgacatcttt
gggcaccacatcaccgcggtacagcaggcaacaagccatgtatttaccatgacgggggtcacatttcaccatctgattggctggctcaaagcaagcattg
gtgatctcggatacagagagctgctcatgataagctttctctgcagagataacaggggcataggtggccagagggaaatggatacgggggtagggtacca
agttggtttggaattctgtcaggtccaca
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: