XB-CLONE-3947260
Clone Name: xthr19O20
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Gene:
sec11c
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN101573 [+]
gcagtgactgagctgccgcccagccccatgcccccggctgcccagcgcctggcatagacacagccaccatggacctgttcggggaccctcggaggatgaa
caagaggcagctctactaccaggttctgaattttgccatgatagtatcttcagctctaatgatatggaaaggcctcatagtggtgaccggaagtgagagt
ccaatagtcgtcgtgctgagtggcagcatggagccggctttccatcggggggatctgctgttcctgacgaacttccaagaagaccccattcgagcaggcg
aaattgtagtgtttaaagtggagggcagagacatcccgattgttcacagggtcatcaaagtgcatgaaaaggagaatggagatatcaaattcctcactaa
aggagataacaatgaagtcgatgaccgaggattatataaggaggggcaaaattggctggaaaagaaagatgttgtggggagagcgcgggggtttttgcca
tatgttggtatggtcactattgtcatgaatgattacccaaaatttaagtatgcggtgttggccattatgggcgcctttgtactgttaaagcgtgaatcct
aacactggaaaaaggcataagaataataaaggcataatatatgact
3' EST: CN101572 [+]ttacattgtattataaaatgtagcatgtacaacggaaccgttatctgaataaataacctaaaggtgacatctagaagaacgtgagttttgtatgtccgga
cagttcccaggtatttgttacgttattacagagaagaaaatacaatatattagtcatatattatgcctttattattcttatgcctttttccagtgttagg
attcacgctttaacagtacaaaggcgcccataatggccaacaccgcatacttaaattttgggtaatcattcatgacaatagtgaccataccaacatatgg
caaaaacccccgcgctctccccacaacatctttcttttccagccaattttgcccctccttatataatcctcggtcatcgacttcattgttatctccttta
gtgaggaatttgatatctccattctccttttcatgcactttgatgaccctgtgaacaatcgggatgtctctgccctccactttaaacactacaatttcgc
ctgctcgaatggggtcttcttggaagttcgtcaggaacagcagatccccccgatggaaagccggctccatgctgccactcagcacgacgactattggact
ctcacttccggtcaccactatgaggcctttccatatcattagagctgaagatactatcatggcaaaattcagaacctggtagtagagctgcctcttgttc
atcctccgagggtccccgaacaggtccatggtggctgtgtctatgccaggcgc
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: