XB-CLONE-3945680
Clone Name: xthr20E16
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Gene:
rpl7a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN102810 [+]
gctttctctgcccctgtccaccatgcctaaagggaagaaggccaaggggaagaaggtggcacccgccccttccgtcgtgaagaaggccgaggccaagaag
gtggtcaaccctctgttcgagaagaggccaaagaacttcggcattggtcaagatatccagcccaagcgtgatctgacccgctttgtcaaatggccccgtt
acatcagactccagcgccagaaggccatcctgtacaagaggctcaaggtcccacctgcaatcaaccagttcacccaagccttggaccgtcagacagccac
acagctcttcaaactggcccacaagtacagacctgagactaaacaggaaaagaagaaaaggctgctggccagggctgagcagaaggccgctggcaaggga
gacgttcccaccaagagaccacctgtactcagagctggtgtcaacactgtcaccacccttgtggaaaacaagaaggctcagcttgttgtgatcgcccatg
atgtcgatcctatcgagctcgtcgtgttcctccctgctttgtgccgcaagatgggagtcccatactgcattgtcaagggcaaggccagactgggccgcct
cgtgcacaggaagacctgcaccagcgtagcactcacacaggttaaccctgaggacaaaggtgcc
3' EST: CN102809 [+]cttggtggccagctcctttgcttttgccttctccagtttggcgatgcgggcaacggacttggggcccaggacaccacctccccagtgacgacggatctca
tcgaatctgtcattgtagttggtcttaactgcttccaccaacttggcgagggcacctttgtcctcagggttaacctgtgtgagtgctacgctggtgcagg
tcttcctgtgcacgaggcggcccagtctggccttgcccttgacaatgcagtatgggactcccatcttgcggcacaaagcagggaggaacacgacgagctc
gataggatcgacatcatgggcgatcacaacaagctgagccttcttgttttccacaagggtggtgacagtgttgacaccagctctgagtacaggtggtctc
ttggtgggaacgtctcccttgccagcggccttctgctcagccctggccagcagccttttcttcttttcctgtttagtctcaggtctgtacttgtgggcca
gtttgaagagctgtgtggctgtctgacggtccaaggcttgggtgaactggttgattgcaggtgggaccttgagcctcttgtacaggatggccttctggcg
ctggagtctgatgtaacggggccatttgacaaagcgggtcagatcacgcttgggctggatatcttgaccaatgccgaagttctttggcctcttctcg
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: