XB-CLONE-3945519
Clone Name: xthr20O12
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Gene:
eif5a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN102916 [+]
gcctttttttttgttggaagtactggtagacacccagcaacatggcagatgatatagatttcaccagtggagatgctggggcttcctctactttccccat
gcagtgctctgctctgcgcaagaatgggtttgtggttctgaaaggacgtccctgtaagattgttgaaatgtcaacttccaagactggcaaacacggtcat
gccaaggtccacctggttggtattgatatctttactggtaagaagtatgaagatatttgtccttctacccacaacatggatgttccaaatattaaaagat
gtgattaccaactgattggtattaatgataattacctgtcccttctgagtgatagtggtgatgttcgtgaggatctgaagatgccagagggagatttggg
aagggaaatactgatgaaacatgatggaggagaggagatcttggtcacagtcttgactgcaatgaatgaagaatgtgctgtagccctcaaggcaatgact
aaataaacagggatcccccaggctggagcaatctgctcaggtcaagataaccacagatcttaaatttggttctgattgtcaccaaagctatggccttcac
aaatgacctcatttccttttgacttgttttgcaattactgctggtttctaaatttggttgttcaagtcaactggcaaccttcaatttcactgcagtttta
aattttgttaatgctt
3' EST: CN102915 [+]ttttttagtctgatgacaaaggcctcgagtgaaaatctgtagcagtaacactcatttgtgccacaaatgcctgctgtgtagtatcacccaacatacagat
tgcctgtctagactgtaggcaggctttgacaccattttataagtttacaatatgcaagccaatgcattggcataggagagtacagaggtaatacaaatta
gttgcacatggccaccaggactaactttttcttttaatatggaggggctgggaaagtggcaaatcaactggcattttaaaagaaaaaaaaaaaaaaaaaa
aaattcactcagtagtccaaagatcttccacactgagatttgaaccatagccaaacatgcataagatttaaacttgctccttaagttaatatccaataat
tctaggtcttcttattttctttacatataagtaaaggtataccaaggtgtttttgtttttaataggacagcccagttgaactacctttgaattctaggtg
ctatttacagattaaaccaaaggcctaattaaaggacatctgtttaatcatgatgcatacagtttagatgctttatatgtttagttggaccctcaaaata
agcaccaatagaaatatgaaaggtaacaaccaaagcattaacaaaatttaaaactgcagtgaaattgaaggttgccagttgacttgaacaaccaaattta
gaaaccagcagtaattgcaaaacaagtcaaaaggaaat
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: