XB-CLONE-3945462
Clone Name: xthr20D05
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Gene:
gsc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN102953 [+]
ggcagtctgcatgaagttttcagccaggagagagagagacaaagtcgcagagtctcctgctttctactcactacccagcagaggattgccccccactaaa
cccagcgctaaggaacccccaaacaggacttgctttccctcctcacacatttgggtgatgccttctggcgtgttcagcattgacaacattttggctgcca
gacctcgctgcagggagtctctctatggccccgcagattacagcggattttataaccgagctgtggccccaacttctactcttcaagcggtgactggatc
taggctgggattcaataactactactatggacagttgcacgtacagacgcctatgggcccctcatgctgcggggcagtgcaaagtctggggacgcagcaa
tgctcctgtgtgccccccgctacagcatatgatggtgcaggatcagtgctgatgcccccagtgcctcaccaaatgctcccttatatgaatgtgggcactt
tgtcacggactgagctacagttgctgaaccagctgcactgcaggaggaagaggaggcacaggaccatcttcactgatgagcaactggaagcactggagaa
tctcttccaagaaaccaagtacccagacgtgggcaccagagaacagctggcaaggagggttcacctcagagaggaaaaagt
3' EST: CN102952 [+]gtacagtgtatctgtgcaaaatgtctgcacattcttttttatttacaggcaatgaaatgataatatttacactttatgtacagatcccacatcatggcac
tgctggggcgttcagctatcccgatgtgcaagtctttagtcttattccagaggaaccaatcaactgtcagagtccaggtcacttttaacctgttcgtccc
ctttctctgttgagttttttgaggacttattccacttttgggtattttctgattcttctgatgaagatcttttctgcctcctccactttgctcttcgatt
tttgaaccaaacctctactttttcctctctgaggtgaaccctccttgccagctgttctctggtgcccacgtctgggtacttggtttcttggaagagattc
tccagtgcttccagttgctcatcagtgaagatggtcctgtgcctcctcttcctcctgcagtgcagctggttcagcaactgtagctcagtccgtgacaaag
tgcccacattcatataagggagcatttggtgaggcactgggggcatcagcactgatcctgcaccatcatatgctgtagcggggggcacacaggagcattg
ctgcgtccccagactttgcactgccccgcagcatgaggggcccataggcgtctgtacgtgcaactgtccatagtagtagttattgaatcccagcct
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: