XB-CLONE-3945294
Clone Name: xthr20N05
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Gene:
actc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN103063 [+]
gagccaatttaatcacagcaaccagctctcctgtgctgaccagaacctaacctgccactatgtgtgacgacgaggagactaccgccttggtgtgcgacaa
tggctccgggctggtgaaggctgggttcgctggggatgatgccccacgtgctgtgttcccctccatcgttggtcgcccccgtcaccagggtgtcatggtg
ggtatgggtcagaaagactcctacgtaggtgatgaagctcagagcaagagaggtatcctgaccctgaagtaccccattgaacacggtatcatcaccaact
gggatgatatggagaagatctggcaccacaccttctacaatgagctgcgtgtggcccctgaggagcaccccaccctgctcactgaggccccactgaaccc
caaggccaaccgtgaaaagatgacacagatcatgtttgagaccttcaacgtcccagccatgtatgttgccatccaggctgtgctgtccctgtacgcttct
ggtcgtaccacaggtatcgttcttgactccggtgatggtgtcacccacaatgtccccatctatgaaggttatgccctgccccatgccatcatgcgtctgg
acctggctggtagagacctcacagattacctcatgaagatcctgactgaacgtggctactcttttgtcaccacagctgaacgtgaaattgtccgtgacat
caaggaaaagctgtgctatgt
3' EST: CN103062 [+]agaaagtataaatatgatggagtgtaacaaactgtacagtgactgtccaggtcttgttttcttcgactttgaggtactttgtatgtgttgcttggaggag
tgtgcgtaatgtttagaagcacttcctgtgcacaatggaggggcctgcctcatcgtattcctgtttgctgatccacatctgttggaaggtggacagggag
gccaagatggagccaccgatccagactgagtatttacgctcaggtggggcaatgatcttgatcttcatggtgctgggagccaaggcagtgatttccttct
gcattctgtcagcaataccagggtacatggtggtaccaccagagaggacattgttggcgtacaggtccttgcggatatcaatgtcgcatttcatgatgct
gttgtaggtagtttcatggataccagcagattccataccaatgaaggaaggctggaagagggtctcagggcagcggaaacgttcatttccaatggtgatg
acctgaccgtcgggaagctcatagctcttctccagggaggaggaagaggcagcggtggccatttcattctcaaagtccaaagccacatagcacagctttt
ccttgatgtcacggacaatttcacgttcagctgtggtgacaaaagagtagccacgttcagtcaggatcttcatg
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: